製品の概要

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: 0.02% Sodium Azide
  • Concentration information loading...
  • 精製度
    Protein A purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab42114 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 5 µg/ml.
WB 1/1000 - 1/15000. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).Can be blocked with Dopamine Receptor D3 peptide (ab128688).
ELISA 1/50000.
Dot blot 1/50000.
IP Use at an assay dependent dilution. 2 µl of this antibody will immunoprecpitate 65-80% dopamine recombinant D3 receptor protein from COS1 cells.

ターゲット情報

  • 機能
    This is one of the five types (D1 to D5) of receptors for dopamine. The activity of this receptor is mediated by G proteins which inhibit adenylyl cyclase. Promotes cell proliferation.
  • 組織特異性
    Brain.
  • 関連疾患
    Genetic variation in DRD3 is associated with essential tremor hereditary type 1 (ETM1) [MIM:190300]. ETM1 is the most common movement disorder. The main feature is postural tremor of the arms. Head, legs, trunk, voice, jaw, and facial muscles also may be involved. The condition can be aggravated by emotions, hunger, fatigue and temperature extremes, and may cause a functional disability or even incapacitation. Inheritance is autosomal dominant.
  • 配列類似性
    Belongs to the G-protein coupled receptor 1 family.
  • 翻訳後修飾
    Phosphorylated by GRK4 (GRK4-alpha and GRK4-gamma).
  • 細胞内局在
    Cell membrane. Both membrane-bound and scattered in the cytoplasm during basal conditions. Receptor stimulation results in the rapid internalization and sequestration of the receptors at the perinuclear area (5 and 15 minutes), followed by the dispersal of the receptors to the membrane (30 minutes). DRD3 and GRK4 co-localize in lipid rafts of renal proximal tubule cells.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 3dopamine receptor antibody
    • D antibody
    • D(3) dopamine receptor antibody
    • Dopamine D3 receptor antibody
    • Dopamine receptor D3 antibody
    • DRD3 antibody
    • DRD3_HUMAN antibody
    • ETM1 antibody
    • FET1 antibody
    see all

画像

  • Anti-Dopamine Receptor D3 antibody (ab42114) at 1/750 dilution + PC-D3R sample

    Predicted band size : 50 kDa
    Observed band size : 50 kDa
  • ICC/IF image of ab42114 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab42114, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

参考文献

This product has been referenced in:
  • Aher YD  et al. A Novel Heterocyclic Compound CE-104 Enhances Spatial Working Memory in the Radial Arm Maze in Rats and Modulates the Dopaminergic System. Front Behav Neurosci 10:20 (2016). WB . Read more (PubMed: 26941626) »
  • Li Y & Kuzhikandathil EV Molecular characterization of individual D3 dopamine receptor-expressing cells isolated from multiple brain regions of a novel mouse model. Brain Struct Funct 217:809-33 (2012). Read more (PubMed: 22286951) »

See all 4 Publications for this product

レビューと Q&A

Thank you for contacting us.

The catalogue number is ab105625.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

All our antibodies are for research purposes only and not for diagnostic or therapeutic ends. We therefore mainly test our products in in vitro application such as WB and IHC. All four antibodies mentioned have not been tested in vivo however this does...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - other (pancreatic islet lysate, membrane fraction)
Loading amount
18 µg
Specification
pancreatic islet lysate, membrane fraction
Gel Running Conditions
Reduced Denaturing (10% gel)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Jan 17 2013

Thank you for your email. I am sorry to hear that you have been experiencing problems with this antibody.

I have read the details you have kindly provided and have following further questions

- What is the problem with these products...

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Finally, it is published, please check the product code ab128688 for blocking peptide.

Many thanks for having patience!

I have forwarded the details to datasheet team. They are now busy in correcting the error and publishing the new datasheet. I will let you know soon, the catalogue number of blocking peptide for ab42114.

I apologize for the delays, we are doi...

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Wehave decidedto publish the datasheet of blocking peptide for ab42114; I will send you the catalogue number soon.

Thank you for contacting us.

We can supply the blocking peptide for AB42114. The price will be £113 for 125ug size. Could you let me know if the customer wishes to but this product, I will then contact the datasheet for publishing this...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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