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Our Abpromise guarantee covers the use of ab40655 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/5000. Stains specific cells in various regions of PLP-fixed rat brain sections including dedritc labelling in the molecular layers as well as the plasma membranes of neurons in layer 5 or cortex and the glomeruli of the olfactory bulb.|
|Flow Cyt||Use at an assay dependent concentration. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.|
|WB||1/500. Detects a band of approximately 50 kDa (predicted molecular weight: 44 kDa).Can be blocked with Dopamine Receptor D3 peptide (ab137997). Blocking with 5% goat or donkey serum significantly reduces background as compared to BSA or milk.|
ab40655 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab40655 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ab40655 has not yet been referenced specifically in any publications.