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Recombinant rat Dopamine Receptor D1, carboxy terminus.
Our Abpromise guarantee covers the use of ab78021 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/250 - 1/500. Predicted molecular weight: 49 kDa.|
|IHC (PFA fixed)||Use at an assay dependent concentration.|
Immunohistochemical analysis of formaldehyde-fixed paraffin-embedded rat cordical tissue sections, labelling Dopamine Receptor D1 with ab78021 at a dilution of 1/500 incubated for 2 hours at 21°C in TBS, BSA & azide diluent. Heat mediated antigen retrival was done via citric acid. Blocking was with 1% BSA incubated for 10 minutes at 21°C. Secondary used was a Goat anti-mouse poyclonal biotin conjugate at 1/300. Image of subventricular region of striatum shows appropriate positivitiy.
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