Anti-Dopamine Receptor D1 抗体 [SG2-D1a] (ab78021)

製品の概要

  • 製品名Anti-Dopamine Receptor D1 antibody [SG2-D1a]
    Dopamine Receptor D1 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [SG2-D1a] to Dopamine Receptor D1
  • アプリケーション適用あり: IHC-P, WB, IHC (PFA fixed)more details
  • 種交差性
    交差種: Mouse, Rat, Sheep
    非交差種: Rabbit, Cow, Dog, Human, Pig
  • 免疫原

    Recombinant rat Dopamine Receptor D1, carboxy terminus.

  • ポジティブ・コントロール
    • Mouse brain lysate; rat caudate tissue.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C.
  • バッファーpH: 7.60
    Preservative: 0.1% Sodium azide
    Constituent: 0.0536% PBS

    Please note that some batches of ab78021 may contain 0.4M arginine. Please contact Scientific Support for further information.
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノモノクローナル
  • クローン名SG2-D1a
  • アイソタイプIgG2b
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab78021 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/100.
WB 1/250 - 1/500. Predicted molecular weight: 49 kDa.
IHC (PFA fixed) Use at an assay dependent concentration.

ターゲット情報

  • 機能Dopamine receptor whose activity is mediated by G proteins which activate adenylyl cyclase.
  • 組織特異性Detected in caudate, nucleus accumbens and in the olfactory tubercle.
  • 配列類似性Belongs to the G-protein coupled receptor 1 family.
  • 細胞内局在Cell membrane. Endoplasmic reticulum membrane. Transport from the endoplasmic reticulum to the cell surface is regulated by interaction with DNAJC14.
  • Information by UniProt
  • 参照データベース
  • 別名
    • D(1A) dopamine receptor antibody
    • D1A dopamine receptor antibody
    • DADR antibody
    • Dopamine D1 receptor antibody
    • dopamine receptor D1 antibody
    • DR D1 antibody
    • DR D1A antibody
    • DRD 1 antibody
    • DRD 1A antibody
    • DRD1 antibody
    • DRD1_HUMAN antibody
    • DRD1A antibody
    see all

Anti-Dopamine Receptor D1 antibody [SG2-D1a] 画像

  • Immunohistochemical analysis of formaldehyde-fixed paraffin-embedded rat cordical tissue sections, labelling Dopamine Receptor D1 with ab78021 at a dilution of 1/500 incubated for 2 hours at 21°C in TBS, BSA & azide diluent. Heat mediated antigen retrival was done via citric acid. Blocking was with 1% BSA incubated for 10 minutes at 21°C. Secondary used was a Goat anti-mouse poyclonal biotin conjugate at 1/300. Image of subventricular region of striatum shows appropriate positivitiy.

    See Abreview

  • Anti-Dopamine Receptor D1 antibody [SG2-D1a] (ab78021) at 2 µg/ml + Brain (Mouse) Tissue Lysate at 20 µg

    Secondary
    Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 49 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 100 kDa,75 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 4 minutes
  • IHC image of ab78021 staining in human normal hippocampus formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH9, epitope retrieval solution 2) for 20 mins. The section was then incubated with ab78021, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-Dopamine Receptor D1 antibody [SG2-D1a] (ab78021) 使用論文

ab78021 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Brain)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization No
Specification Brain
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Fixative Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

投稿 Sep 21 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (Brain)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization No
Specification Brain
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Fixative Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

投稿 Sep 21 2015

Application Western blot
Loading amount 50 µg
Gel Running Conditions Non-reduced Non-Denaturing (Native) (stacking gel: 4%, resolving gel: 5-13%)
Sample Rat Tissue lysate - whole (hippocampi total membrane fraction)
Specification hippocampi total membrane fraction
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Ms. Sunetra Jitkar

Verified customer

投稿 Feb 19 2014

Application Immunohistochemistry (Frozen sections)
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
Sample Mouse Tissue sections (Hippocampus)
Specification Hippocampus
Permeabilization No
Fixative Formaldehyde
Username

Abcam user community

Verified customer

投稿 Feb 10 2014

Thank you for confirming that information. I have organised for ab98790 to be sent out to you (Order number xxxxx, Purchase order numberFOCR xxxxx). As we have the Easter bank holidays this will unfortunately only reach you on Wednesday next week.
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Thank you for getting back to me.

I can arrange for a different secondary antibody to be sent to you. In order to organise this I will need the order numbers of ab78021 and ab97040, or the approximate delivery date and delivery address.
...

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Thank you for contacting us.


As discussed over the phone, the dilution factors stated on the datasheet are recommended starting points only. Depending on the sample you are using and the protocol being used you may need to increase or d...

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Thank you for contacting us. The protocol is, for the most part, standard. The instruction to not boil the samples is referring to the tendency of multi-pass membrane proteins to aggregate if boiled. To denature your samples before loading into the...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"