製品の概要

  • 製品名
    Anti-Dopamine D2 Receptor antibody
    Dopamine D2 Receptor 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Dopamine D2 Receptor
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Rat, Human
    交差が予測される動物種: Mouse, Chimpanzee, Monkey
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Dopamine D2 Receptor.

    (Peptide available as ab97357.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the following tissue lysates: Human brain; Human skeletal muscle; Rat Substantia nigra; Rat Dopaminergic nucleus.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab85367 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 49 kDa (predicted molecular weight: 50 kDa).

ターゲット情報

  • 機能
    This is one of the five types (D1 to D5) of receptors for dopamine. The activity of this receptor is mediated by G proteins which inhibit adenylyl cyclase.
  • 関連疾患
    Defects in DRD2 are associated with dystonia type 11 (DYT11) [MIM:159900]; also known as alcohol-responsive dystonia. DYT11 is a myoclonic dystonia. Dystonia is defined by the presence of sustained involuntary muscle contractions, often leading to abnormal postures. DYT11 is characterized by involuntary lightning jerks and dystonic movements and postures alleviated by alcohol. Inheritance is autosomal dominant. The age of onset, pattern of body involvement, presence of myoclonus and response to alcohol are all variable.
  • 配列類似性
    Belongs to the G-protein coupled receptor 1 family.
  • 細胞内局在
    Cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • D(2) dopamine receptor antibody
    • D2 dopamine receptor antibody
    • D2DR antibody
    • D2R antibody
    • Dopamine D2 receptor antibody
    • Dopamine receptor D2 antibody
    • DRD 2 antibody
    • DRD2 antibody
    • DRD2_HUMAN antibody
    see all

画像

  • All lanes : Anti-Dopamine D2 Receptor antibody (ab85367) at 1 µg/ml

    Lane 1 : Human brain tissue lysate - total protein (ab29466)
    Lane 2 : Human skeletal muscle tissue lysate - total protein (ab29330)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 50 kDa
    Observed band size : 49 kDa (why is the actual band size different from the predicted?)


    Exposure time : 2 minutes
  • All lanes : Anti-Dopamine D2 Receptor antibody (ab85367) at 1 µg/ml

    Lane 1 : Rat Substantia Nigra Lysate
    Lane 2 : Dopaminergic nucleus Rat Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) (ab65484) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 50 kDa
    Observed band size : 49 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 52 kDa (possible glycosylated form).

    Exposure time : 4 minutes

プロトコール

参考文献

This product has been referenced in:
  • Obici S  et al. Moderate voluntary exercise attenuates the metabolic syndrome in melanocortin-4 receptor-deficient rats showing central dopaminergic dysregulation. Mol Metab 4:692-705 (2015). WB . Read more (PubMed: 26500841) »
  • Lewitus GM  et al. An adaptive role of TNFa in the regulation of striatal synapses. J Neurosci 34:6146-55 (2014). Read more (PubMed: 24790185) »

See all 2 Publications for this product

レビューと Q&A

Thank you for contacting us.  Unfortunately, I was unable to locate any amino acid sequences for Tilapia S100, S100 beta, or Dopamine D2 Receptor with which to compare the immunogen sequences for these antibodies.  For this reason, we have no informati...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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