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Our Abpromise guarantee covers the use of ab21218 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent concentration. PubMed: 18261457|
|WB||1/800. Detects a band of approximately 48-51 kDa.Can be blocked with Dopamine D2 Receptor peptide (ab219840).
We recommend incubating with the antibody for 1h if the background is high as well as diluting the antibody further. Also blocking with non-bovine products (e.g. goat milk, normal goat serum) is recommended. Use of cow milk or BSA is not preferred.
Dilution optimised using chromogenic detection, and we recommend diluting further when using chemiluminescent substrates.
|ELISA||Use at an assay dependent concentration.|
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