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Recombinant protein fragment corresponding to a region within amino acids 8 and 290 of Dopamine beta Hydroxylase (NP_000778).
Our Abpromise guarantee covers the use of ab96615 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 69 kDa.|
|IHC-P||1/100 - 1/500.|
|IHC-Fr||Use at an assay dependent concentration.|
Separated by 7.5% SDS-PAGE.
ab96615 staining Dopamine beta Hydroxylase in human colorectal tumor tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with formaldehyde, permeabilized with 0.3% Triton X-100 in PBS and blocked with 5% goat serum for 1 hour at 24°C. Samples were incubated with primary antibody (1/1000) for 3 hours at 24°C. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG monoclonal (1/2000) was used as the secondary antibody.