Anti-Dopamine beta Hydroxylase 抗体 (ab96615)

製品の概要

  • 製品名
    Anti-Dopamine beta Hydroxylase antibody
    Dopamine beta Hydroxylase 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Dopamine beta Hydroxylase
  • アプリケーション
    適用あり: WB, IHC-P, IHC-Frmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant protein fragment corresponding to a region within amino acids 8 and 290 of Dopamine beta Hydroxylase (NP_000778).

  • ポジティブ・コントロール
    • WB: SH-SY5Y postive control, 293T negative control IHC-P: SW480 xenograft

法規制情報

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab96615 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/3000. Predicted molecular weight: 69 kDa.
IHC-P 1/100 - 1/500.
IHC-Fr Use at an assay dependent concentration.

ターゲット情報

  • 機能
    Conversion of dopamine to noradrenaline.
  • パスウェイ
    Catecholamine biosynthesis; (R)-noradrenaline biosynthesis; (R)-noradrenaline from dopamine: step 1/1.
  • 関連疾患
    Defects in DBH are the cause of dopamine beta-hydroxylase deficiency (DBH deficiency) [MIM:223360]; also known as norepinephrine deficiency or noradrenaline deficiency. This disorder is characterized by profound deficits in autonomic and cardiovascular function, but apparently only subtle signs, if any, of central nervous system dysfunction.
  • 配列類似性
    Belongs to the copper type II ascorbate-dependent monooxygenase family.
    Contains 1 DOMON domain.
  • 細胞内局在
    Cytoplasmic vesicle > secretory vesicle lumen. Cytoplasmic vesicle > secretory vesicle > chromaffin granule lumen and Cytoplasmic vesicle > secretory vesicle membrane. Cytoplasmic vesicle > secretory vesicle > chromaffin granule membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • dbh antibody
    • DBM antibody
    • Dopamine beta hydroxylase antibody
    • Dopamine beta monooxygenase antibody
    • Dopamine beta-hydroxylase (dopamine beta-monooxygenase) antibody
    • Dopamine beta-monooxygenase antibody
    • DOPO_HUMAN antibody
    • OTTHUMP00000022501 antibody
    • Soluble dopamine beta-hydroxylase antibody
    see all

Anti-Dopamine beta Hydroxylase antibody 画像

  • All lanes : Anti-Dopamine beta Hydroxylase antibody (ab96615) at 1/2000 dilution

    Lane 1 : SH-SY5Y whole cell extract
    Lane 2 : 293T whole cell extract

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 69 kDa

    Separated by 7.5% SDS-PAGE.

  • mmunohistochemical analysis of Dopamine beta Hydroxylase in paraffin embedded SW480 xenograft, using ab96615 at a 1/500 dilution.
  • ab96615 staining Dopamine beta Hydroxylase in human colorectal tumor tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with formaldehyde, permeabilized with 0.3% Triton X-100 in PBS and blocked with 5% goat serum for 1 hour at 24°C. Samples were incubated with primary antibody (1/1000) for 3 hours at 24°C. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG monoclonal (1/2000) was used as the secondary antibody.

    See Abreview

Anti-Dopamine beta Hydroxylase antibody (ab96615) 使用論文

This product has been referenced in:
  • Liu Q  et al. Human neural crest stem cells derived from human ESCs and induced pluripotent stem cells: induction, maintenance, and differentiation into functional schwann cells. Stem Cells Transl Med 1:266-78 (2012). ICC/IF ; Human . Read more (PubMed: 23197806) »

See 1 Publication for this product

Product Wall

Application
Immunohistochemistry (Frozen sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Sample
Human Tissue sections (Colorectal tumors)
Specification
Colorectal tumors
Permeabilization
Yes - 1xPBS+0.3%t-X-100
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

投稿 Oct 09 2014

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this...

Read More

Thank you for taking time to complete our questionnaire. I am sorry to hear that this antibody is not providing satisfactory results.

The details provided will enable us to investigate this case and will provide us with vital information for...

Read More

Thank you for contacting us.

I am sorry to hear you are experiencing difficulties with one of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

登録