Anti-Dopamine beta Hydroxylase 抗体 (ab96615)


  • 製品名Anti-Dopamine beta Hydroxylase antibody
    Dopamine beta Hydroxylase 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Dopamine beta Hydroxylase
  • アプリケーション適用あり: WB, IHC-P, ICC/IF, IHC-Frmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant protein fragment corresponding to a region within amino acids 8 and 290 of Dopamine beta Hydroxylase (NP_000778).

  • ポジティブ・コントロール
    • WB: 293T, A431, H1299, HeLa, HepG2, MOLT4 and Raji cell lysates ICC/IF: HeLa cell IHC-P: SW480 xenograft





Our Abpromise guarantee covers the use of ab96615 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/3000. Predicted molecular weight: 69 kDa.
IHC-P 1/100 - 1/500.
ICC/IF 1/100 - 1/200.
IHC-Fr Use at an assay dependent concentration.


  • 機能Conversion of dopamine to noradrenaline.
  • パスウェイCatecholamine biosynthesis; (R)-noradrenaline biosynthesis; (R)-noradrenaline from dopamine: step 1/1.
  • 関連疾患Defects in DBH are the cause of dopamine beta-hydroxylase deficiency (DBH deficiency) [MIM:223360]; also known as norepinephrine deficiency or noradrenaline deficiency. This disorder is characterized by profound deficits in autonomic and cardiovascular function, but apparently only subtle signs, if any, of central nervous system dysfunction.
  • 配列類似性Belongs to the copper type II ascorbate-dependent monooxygenase family.
    Contains 1 DOMON domain.
  • 細胞内局在Cytoplasmic vesicle > secretory vesicle lumen. Cytoplasmic vesicle > secretory vesicle > chromaffin granule lumen and Cytoplasmic vesicle > secretory vesicle membrane. Cytoplasmic vesicle > secretory vesicle > chromaffin granule membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • dbh antibody
    • DBM antibody
    • Dopamine beta hydroxylase antibody
    • Dopamine beta monooxygenase antibody
    • Dopamine beta-hydroxylase (dopamine beta-monooxygenase) antibody
    • Dopamine beta-monooxygenase antibody
    • DOPO_HUMAN antibody
    • OTTHUMP00000022501 antibody
    • Soluble dopamine beta-hydroxylase antibody
    see all

Anti-Dopamine beta Hydroxylase antibody 画像

  • Anti-Dopamine beta Hydroxylase antibody (ab96615) at 1/5000 dilution + HepG2 whole cell lysate at 30 µg

    Predicted band size : 69 kDa
  • Immunofluorescence analysis of Dopamine beta Hydroxylase in paraformaldehyde fixed HeLa cell, using ab96615 at a 1/200 dilution.
    Lower image: merged with DNA probe.
  • mmunohistochemical analysis of Dopamine beta Hydroxylase in paraffin embedded SW480 xenograft, using ab96615 at a 1/500 dilution.
  • ab96615 staining Dopamine beta Hydroxylase in human colorectal tumor tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with formaldehyde, permeabilized with 0.3% Triton X-100 in PBS and blocked with 5% goat serum for 1 hour at 24°C. Samples were incubated with primary antibody (1/1000) for 3 hours at 24°C. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG monoclonal (1/2000) was used as the secondary antibody.

    See Abreview

Anti-Dopamine beta Hydroxylase antibody (ab96615) 使用論文

This product has been referenced in:
  • Liu Q  et al. Human neural crest stem cells derived from human ESCs and induced pluripotent stem cells: induction, maintenance, and differentiation into functional schwann cells. Stem Cells Transl Med 1:266-78 (2012). ICC/IF ; Human . Read more (PubMed: 23197806) »

See 1 Publication for this product

Product Wall

Application Immunohistochemistry (Frozen sections)
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Sample Human Tissue sections (Colorectal tumors)
Specification Colorectal tumors
Permeabilization Yes - 1xPBS+0.3%t-X-100
Fixative Formaldehyde

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投稿 Oct 09 2014

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