製品の概要

  • 製品名
    Anti-Dopamine antibody [2B11]
    Dopamine 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [2B11] to Dopamine
  • 特異性
    This antibody detects catecholamine Affinity of this antibody: Kd = 10-8 for DA-BSA Kd = 10-7 for NA-BSA Kd = 10-4 for unconjugated DA
  • アプリケーション
    適用あり: ICC, Neutralisingmore details
  • 免疫原

    Chemical/ Small Molecule conjugated to BSA.

  • 特記事項


    Detection of catecholamine-secreting cells.

    Abcam is committed to meeting high standards of ethical manufacturing and as such, we will be discontinuing this product, which has been generated by the ascites method, within the next year. We are sorry for any inconvenience this may cause. If you would like help finding an alternative product, please do not hesitate to contact our scientific support team.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: 0.1% Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • 精製度
    IgG fraction
  • 一次抗体 備考
    Detection of catecholamine-secreting cells.
  • ポリ/モノ
    モノクローナル
  • クローン名
    2B11
  • ミエローマ
    Sp2/0
  • アイソタイプ
    IgG1
  • 軽鎖の種類
    unknown
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab8281 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC Use at an assay dependent concentration.
Neutralising Use at an assay dependent concentration.

ターゲット情報

  • 関連性
    Dopamine (C6H3OH)2-CH2CH2NH2)is a catecholamine neurotransmitter in the brain. Its chemical name is 4-(2-aminoethyl)benzene-1,2-diol. Dopamine is a hormone released by the hypothalamus. Its main function is to inhibit the release of prolactin from the anterior lobe of the pituitary. It can be used as a sympathomimetic drug, producing effects such as increased heart rate and blood pressure.
  • 細胞内局在
    Secreted
  • 別名
    • 3 4 dihydroxyphenethylamine antibody
    • 3 hydroxytyramine antibody
    • DA antibody
    • Dopamine antibody
    • Oxytyramine antibody
    see all

Anti-Dopamine antibody [2B11] (ab8281) 使用論文

This product has been referenced in:
  • Baldrich E & Muñoz FX Carbon nanotube wiring: a tool for straightforward electrochemical biosensing at magnetic particles. Anal Chem 83:9244-50 (2011). Read more (PubMed: 22040046) »

See 1 Publication for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Leukemia cells)
Permeabilization
Yes - 0.5% Triton-X100
Specification
Leukemia cells
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

投稿 Jun 29 2017

Thank you for your enquiry. Yes, since the primary antibody was raised in mouse, this secondary will recognize it. I hope this information helps, please do not hesitate to contact us if you need any more advice or information,

Dopamine conjugated with BSA has been used as the immunogen. However, the antibody is not conjugated.

According to literature data dopamine cross-links to some intracellular component during fixation/permeabilization and it remains inside the cell.

This antibody was raised against human dopamine, cross reactivity with other species has not yet been tested.

1% BSA solutions should be uses as normal as a blocking agent in IHC for both the primary and the secondary antibody. The immunogen was a dopamine-BSA conjugate, BSA faciltates the immune response.

This antibody is a mouse monoclonal (IgG1) to human dopamine, not to the D-2 receptor. As far as we are aware, the antibody has not yet been tested in mouse.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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