製品の概要

  • 製品名
    Anti-Dopamine antibody [2B11]
    Dopamine 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [2B11] to Dopamine
  • 特異性
    The affinity of this antibody for dopamine-BSA is 10 nM and for noradrenaline-BSA is 100 nM. Affinity for unconjugated catecholamines(dopamine, noradrenaline, adrenaline) is lower (approx. 100 µM). At 1 uM, this antibody inhibits dopamine binding to membrane receptor from human corpus striatum. Cross-reactivity (%): Dopamine = 100% Noradrenaline = <15% Adrenaline = <10% Normetanephrine = 0% 3-methoxy-tyramine = 0% L-DOPA = 0% Homovanillic acid = 0% Vanillic acid = 0% Vanillylmandelic acid = 0% 4-hydroxy-3-methoxyphenyl glycol = 0% 3,4-dihydroxyphenylacetic acid = 0% Tyramine = 0% beta-phenylethylamine = 0% Amphetamine = 0%
  • アプリケーション
    適用あり: Blocking, IHC-P, IHC-Frmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Full length protein corresponding to Dopamine conjugated to Bovine Serum Albumin (BSA). Immunogen = Dopamine conjugated to bovine serum albumin.

  • 特記事項

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by July 2018 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. 

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    pH: 7.40
    Preservative: 0.1% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • 精製度
    Protein A purified
  • ポリ/モノ
    モノクローナル
  • クローン名
    2B11
  • アイソタイプ
    IgG1
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab1001 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Blocking Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.

ターゲット情報

  • 関連性
    Dopamine (C6H3OH)2-CH2CH2NH2)is a catecholamine neurotransmitter in the brain. Its chemical name is 4-(2-aminoethyl)benzene-1,2-diol. Dopamine is a hormone released by the hypothalamus. Its main function is to inhibit the release of prolactin from the anterior lobe of the pituitary. It can be used as a sympathomimetic drug, producing effects such as increased heart rate and blood pressure.
  • 細胞内局在
    Secreted
  • 別名
    • 3 4 dihydroxyphenethylamine antibody
    • 3 hydroxytyramine antibody
    • DA antibody
    • Dopamine antibody
    • Oxytyramine antibody
    see all

画像

  • ab1001 staining human adrenal tissue sections by IHC-P.  Sections were formaldehyde fixed and subjected to heat mediated antigen retrieval in citrate buffer (pH 6) prior to blocking in 1% serum for 30 minutes at 22°C.  The primary antibody was diluted 1/1000 and incubated with the sample for 30 minutes at 22°C.  A HRP conjugated goat anti-mouse antibody diluted 1/400 was used as the secondary.

    See Abreview

プロトコール

参考文献

This product has been referenced in:
  • Rowell C  et al. Chemoprevention of breast cancer, proteomic discovery of genistein action in the rat mammary gland. J Nutr 135:2953S-2959S (2005). Read more (PubMed: 16317154) »

See 1 Publication for this product

レビューと Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (adrenal)
Specification
adrenal
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate Buffer pH 6.0
Permeabilization
No
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 22°C
Username

Abcam user community

Verified customer

投稿 Oct 05 2007

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (adrenal)
Specification
adrenal
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer pH 6.0
Permeabilization
No
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 22°C
Username

Abcam user community

Verified customer

投稿 Oct 05 2007

Thank you for sending this enquiry because it helped us to find a typographical error in our data sheet. The affinity of this antibody for dopamine-BSA should be 10 nM or 10(-8) M (the data sheet says 10 mM), and the affinity for noradrenaline-BSA shou...

Read More

This antibody should work in IHC on paraformaldehyde-fixed tissue without glutaraldehyde. However, we do not have any information on how well this antibody will work on macaca brain sections.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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