製品の概要

  • 製品名
    Anti-DOPA Decarboxylase antibody
    DOPA Decarboxylase 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to DOPA Decarboxylase
  • アプリケーション
    適用あり: ICC, IHC-Fr, IHC-P, WB, ICC/IF, IHC-FoFrmore details
  • 種交差性
    交差種: Mouse, Rat
  • 免疫原

    SDS denatured, recombinant bovine aromatic L-amino acid decarboxylase expressed in E. coli and purified from inclusion bodies

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab3905 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
WB Use a concentration of 0.1 - 0.5 µg/ml.
ICC/IF Use at an assay dependent concentration. PubMed: 20374418
IHC-FoFr Use at an assay dependent concentration.

ターゲット情報

  • 機能
    Catalyzes the decarboxylation of L-3,4-dihydroxyphenylalanine (DOPA) to dopamine, L-5-hydroxytryptophan to serotonin and L-tryptophan to tryptamine.
  • パスウェイ
    Catecholamine biosynthesis; dopamine biosynthesis; dopamine from L-tyrosine: step 2/2.
  • 関連疾患
    Defects in DDC are the cause of aromatic L-amino-acid decarboxylase deficiency (AADCD) [MIM:608643]. AADCD deficiency is an inborn error in neurotransmitter metabolism that leads to combined serotonin and catecholamine deficiency. It causes developmental and psychomotor delay, poor feeding, lethargy, ptosis, intermittent hypothermia, gastrointestinal disturbances. The onset is early in infancy and inheritance is autosomal recessive.
  • 配列類似性
    Belongs to the group II decarboxylase family.
  • Information by UniProt
  • 参照データベース
  • 別名
    • AADC antibody
    • Aromatic L Amino Acid Decarboxylase antibody
    • Aromatic-L-amino-acid decarboxylase antibody
    • DDC antibody
    • DDC_HUMAN antibody
    • DOPA decarboxylase (aromatic L-amino acid decarboxylase) antibody
    • DOPA decarboxylase antibody
    see all

Anti-DOPA Decarboxylase antibody 画像

  • Anti-DOPA Decarboxylase antibody (ab3905) + Mouse cerebellum lysate at 0.2 mg/ml

    Performed under reducing conditions.
  • Anti-DOPA Decarboxylase antibody (ab3905) + Rat adrenal medulla lysate
  • ab3905 staining DOPA Decarboxylase in rat brain tissue sections by Immunohistochemistry (frozen sections). Tissue was paraformaldehyde fixed, then blocked with 5% serum for 1 hour at 25°C. Primary anitbody used at a 1/500 dilution and incubated for 16 hours at 25°C. The secondary was used at 1/200 goat anti-rabbit IgG conjugated to Alexa Fluor 488 (green).

    See Abreview

  • ab3905 staining DOPA Decarboxylase in mouse midbrain tissue sections by Immunohistochemistry (paraffin embedded sections). Tissue was fixed with paraformaldehyde and a heat mediated antigen retrieval step was performed using 10mM citrate buffer pH 6.0. Samples were then blocked with 1% serum for 30 minutes at 24°C followed by incubation with the primary antibody, at a 1/250 dilution, for 16 hour at 4°C. An Alexa Fluor® 488 conjugated goat anti-rabbit IgG (green) was used as secondary antibody at a 1/250 dilution.

    See Abreview

Anti-DOPA Decarboxylase antibody (ab3905) 使用論文

This product has been referenced in:
  • Xenias HS  et al. Are striatal tyrosine hydroxylase interneurons dopaminergic? J Neurosci 35:6584-99 (2015). IHC ; Mouse . Read more (PubMed: 25904808) »
  • Wienecke J  et al. Spinal cord injury enables aromatic L-amino acid decarboxylase cells to synthesize monoamines. J Neurosci 34:11984-2000 (2014). Read more (PubMed: 25186745) »

See all 12 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Sample
Fruit fly (Drosophila melanogaster) Cell (central nervous system (ventral nerve cord))
Specification
central nervous system (ventral nerve cord)
Permeabilization
Yes - 0.2% Triton-X
Fixative
Formaldehyde
Username

shinya yamamoto

Verified customer

投稿 Jun 13 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Brain)
Specification
Brain
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Nov 16 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Midbrain section)
Specification
Midbrain section
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate buffer pH 6.0
Permeabilization
No
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 24°C
Username

Abcam user community

Verified customer

投稿 Mar 26 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Rat Tissue sections (Brain)
Specification
Brain
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Dec 14 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Rat Tissue sections (Brain)
Specification
Brain
Fixative
Paraformaldehyde
Antigen retrieval step
None
Permeabilization
Yes - 0.1% triton x-100
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
Username

Abcam user community

Verified customer

投稿 Aug 01 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (young postnatal mouse brain)
Specification
young postnatal mouse brain
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.2% Triton X-100
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Sep 21 2007

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Brain slices (40um thick sections))
Specification
Brain slices (40um thick sections)
Fixative
Paraformaldehyde
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10%
Username

Abcam user community

Verified customer

投稿 Mar 13 2006

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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