製品の概要

  • 製品名Donkey Anti-Sheep IgG H&L (HRP)
    IgG 二次抗体 製品一覧
  • 製品の詳細
    Donkey polyclonal Secondary Antibody to Sheep IgG - H&L (HRP)
  • ターゲット生物種Sheep
  • 特異性By immunoelectrophoresis and ELISA this antibody reacts specifically with Sheep IgG and with light chains common to other Sheep immunoglobulins. No antibody was detected against non immunoglobulin serum proteins.
  • アプリケーション適用あり: ICC, IHC-P, ELISA, WBmore details
  • 免疫原

    Other Immunogen Type corresponding to Sheep IgG.

  • 標識HRP

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C.
  • バッファーPreservative: 0.1% Proclin
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP).
  • 特記事項(標識)Molar enzyme/ antibody protein ratio is 4:1
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab97125 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC Use at an assay dependent dilution.
IHC-P 1/200 - 1/5000.
ELISA 1/10000 - 1/100000. (primary)
WB 1/5000 - 1/50000. Colorimetric: 1/500 - 1/30000, Chemiluminescent: 1/10000 - 1/50000

Donkey Anti-Sheep IgG H&L (HRP) (ab97125) 使用論文

This product has been referenced in:
  • Koch F  et al. Metabolic Response to Heat Stress in Late-Pregnant and Early Lactation Dairy Cows: Implications to Liver-Muscle Crosstalk. PLoS One 11:e0160912 (2016). WB . Read more (PubMed: 27513961) »
  • White CI  et al. Cardiomyocyte and Vascular Smooth Muscle-Independent 11ß-Hydroxysteroid Dehydrogenase 1 Amplifies Infarct Expansion, Hypertrophy, and the Development of Heart Failure After Myocardial Infarction in Male Mice. Endocrinology 157:346-57 (2016). WB . Read more (PubMed: 26465199) »

See all 3 Publications for this product

Product Wall

Abreviews
Application Western blot
Rat olfactory epithelium tissue lysate, 10ug/lane
Blocked overnight in 5% nonfat milk
GAP43 1/2000, 1hr in TBS+0.1%Tween
Donkey anti-sheep 1/5000 in 3.5%BSA/TBS+-.1%Tween
Lane1: GAP43 primary antibody, Lane 2: Secondary only negative control

There is a general high background to the secondary but seems to be improved with secondary incubation in BSA and extended washing after secondary incubation.
Username

Abcam user community

Verified customer

投稿 Jul 14 2015

Gracias por su respuesta. Un anticuerpo secundario válido para usar con el primario ab64544 podría ser el ab97125: http://www.abcam.com/Donkey-polyclonal-Secondary-Antibody-to-Sheep-IgG-H-L-HRP-ab97125.html está conjugado con peroxidasa y es apropia...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"