製品の概要

  • 製品名Donkey Anti-Sheep IgG H&L (Biotin)
    IgG 二次抗体 製品一覧
  • ターゲット生物種Sheep
  • アプリケーション適用あり: ICC/IF, IHC-P, IHC-Fr, WB, ELISA, Immunomicroscopy, Dot blotmore details
  • 免疫原

    Full length native Sheep IgG (purified).

  • 標識Biotin

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 0.01% Sodium Azide
    Constituents: 10mg/ml BSA, 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2
  • Concentration information loading...
  • 精製度IgG fraction
  • 特記事項(精製)This product was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities
  • 特記事項(標識)Biotin/Protein Ratio: about 6 per IgG molecule
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab6899 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF
IHC-P
IHC-Fr
WB
ELISA
Immunomicroscopy
Dot blot
  • 追加情報Dot: Use at an assay dependant dilution.
    ELISA: 1/30000 - 1/130000.
    IHC-P: Use at an assay dependant dilution.
    IHC-Fr: Use at an assay dependant dilution.
    IM: Use at an assay dependant dilution.
    WB: Use at an assay dependant dilution.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Donkey Anti-Sheep IgG H&L (Biotin) (ab6899) 使用論文

    This product has been referenced in:
    • Wienecke J  et al. Spinal cord injury enables aromatic L-amino acid decarboxylase cells to synthesize monoamines. J Neurosci 34:11984-2000 (2014). Read more (PubMed: 25186745) »

    See 1 Publication for this product

    Product Wall

    Merci pour votre appel et votre patience.

    Je viens d'avoir la confirmation du laboratoire que effectivement le ratio de conjugaison de la biotine était de ˜6 moles de biotine par mole de IgG.

    Je suis désolée pou...

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    Thank you for your enquiry. We have yet to receive reviews from customers who have purchased and tested this antibody in WB or IHC. We also, don't have the same product with a fluorescent conjugate. To view a full list of our secondary antibodies, you ...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"