Donkey Anti-Rat IgG H&L (DyLight® 488) preadsorbed (ab102260)
Key features and details
- Donkey Anti-Rat IgG H&L (DyLight® 488) preadsorbed
- Conjugation: DyLight® 488. Ex: 493nm, Em: 518nm
- Host species: Donkey
- Isotype: IgG
- Suitable for: Flow Cyt, ICC, IHC-P
Related conjugates and formulations
製品の概要
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製品名
Donkey Anti-Rat IgG H&L (DyLight® 488) preadsorbed
IgG 二次抗体 製品一覧 -
由来種
Donkey -
ターゲット生物種
Rat -
特異性
By immunoelectrophoresis and ELISA this antibody reacts specifically with rat IgG and with light chains common to other rat immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to bovine, chicken, goat, horse, human, mouse, rabbit and sheep IgG was detected. This antibody may cross react with IgG from other species. -
アプリケーション
適用あり: Flow Cyt, ICC, IHC-Pmore details -
吸着処理血清
Chicken, Cow, Goat, Horse, Human, Mouse, Rabbit, Sheep more details -
標識
DyLight® 488. Ex: 493nm, Em: 518nm
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: PBS, 0.2% BSA -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
Antiserum was cross adsorbed using bovine, chicken, goat, horse, human, mouse, rabbit and sheep immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 488. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab102260の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
IF |
1/50 - 1/500.
|
|
Flow Cyt |
1/50 - 1/200.
|
|
ICC |
1/50 - 1/500.
|
|
IHC-P |
1/50 - 1/500.
|
特記事項 |
---|
IF
1/50 - 1/500. |
Flow Cyt
1/50 - 1/200. |
ICC
1/50 - 1/500. |
IHC-P
1/50 - 1/500. |
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (4)
ab102260 は 4 報の論文で使用されています。
- Flores I et al. Myeloid cell-mediated targeting of LIF to dystrophic muscle causes transient increases in muscle fiber lesions by disrupting the recruitment and dispersion of macrophages in muscle. Hum Mol Genet 31:189-206 (2021). PubMed: 34392367
- Welc SS et al. Differential Effects of Myeloid Cell PPARd and IL-10 in Regulating Macrophage Recruitment, Phenotype, and Regeneration following Acute Muscle Injury. J Immunol 205:1664-1677 (2020). PubMed: 32817369
- Welc SS et al. Targeting a therapeutic LIF transgene to muscle via the immune system ameliorates muscular dystrophy. Nat Commun 10:2788 (2019). PubMed: 31243277
- Cottle DL et al. c-MYC-induced sebaceous gland differentiation is controlled by an androgen receptor/p53 axis. Cell Rep 3:427-41 (2013). PubMed: 23403291