Donkey Anti-Rat IgG H&L (Biotin) preadsorbed (ab102259)
Key features and details
- Donkey Anti-Rat IgG H&L (Biotin) preadsorbed
- Conjugation: Biotin
- Host species: Donkey
- Isotype: IgG
- Suitable for: ICC, IHC-P, ELISA, WB
Related conjugates and formulations
製品の概要
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製品名
Donkey Anti-Rat IgG H&L (Biotin) preadsorbed
IgG 二次抗体 製品一覧 -
由来種
Donkey -
ターゲット生物種
Rat -
特異性
By immunoelectrophoresis and ELISA this antibody reacts specifically with rat IgG and with light chains common to other rat immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to bovine, chicken, goat, horse, human, mouse, rabbit and sheep IgG was detected. This antibody may cross react with IgG from other species. -
アプリケーション
適用あり: ICC, IHC-P, ELISA, WBmore details -
吸着処理血清
Chicken, Cow, Goat, Horse, Human, Mouse, Rabbit, Sheep more details -
標識
Biotin
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.2% BSA, PBS -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
Antiserum was cross adsorbed using bovine, chicken, goat, horse, human, mouse, rabbit and sheep immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Biotin. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab102259の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
ICC |
1/200 - 1/1000.
|
|
IHC-P |
1/500 - 1/5000.
|
|
ELISA |
1/10000 - 1/200000. (Primary ELISA).
|
|
WB |
1/10000 - 1/200000. (Chemiluminescent).
|
特記事項 |
---|
ICC
1/200 - 1/1000. |
IHC-P
1/500 - 1/5000. |
ELISA
1/10000 - 1/200000. (Primary ELISA). |
WB
1/10000 - 1/200000. (Chemiluminescent). |
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (2)
ab102259 は 2 報の論文で使用されています。
- Iguchi T et al. Mutually Repulsive EphA7-EfnA5 Organize Region-to-Region Corticopontine Projection by Inhibiting Collateral Extension. J Neurosci 41:4795-4808 (2021). PubMed: 33906900
- Klein S et al. Novel Rat Model of Repetitive Portal Venous Embolization Mimicking Human Non-Cirrhotic Idiopathic Portal Hypertension. PLoS One 11:e0162144 (2016). PubMed: 27589391