Donkey Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97085)

製品の概要

  • 製品名
    Donkey Anti-Rabbit IgG H&L (HRP) preadsorbed
    IgG 二次抗体 製品一覧
  • 製品の詳細
    Donkey polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed
  • ターゲット生物種
    Rabbit
  • 特異性
    By immunoelectrophoresis and ELISA this antibody reacts specifically with Rabbit IgG and with light chains common to other Rabbit immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Less than 0.1% cross reactivity to bovine, chicken, goat, horse, human, mouse, pig and rat was detected.
  • アプリケーション
    適用あり: ICC, IHC-P, WB, ELISAmore details
  • 吸着処理血清

    Chicken, Cow, Goat, Human, Mouse, Pig, Rat more details
  • 標識
    HRP

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C.
  • バッファー
    Preservative: 0.1% Proclin
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    Antiserum was cross adsorbed using bovine, chicken, goat, human, mouse, pig and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP).
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab97085 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC Use at an assay dependent dilution.
IHC-P 1/200 - 1/5000.
WB 1/5000 - 1/50000. (Colorimetric: 1/5000 - 1/30000; Chemiluminescent: 1/10000 - 1/50000)
ELISA 1/10000 - 1/100000. (Primary ELISA)

画像

  • ab134047 staining VCAM1 in mouse aorta tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with methanol and blocked with 10% normal donkey serum for 2 hours at room temperature. Samples were incubated with primary antibody (1/200 in PBS + 0.1% Triton + 2% normal donkey serum) for 12 hours at 4°C. A HRP-conjugated donkey anti-rabbit IgG H&L (ab97085) (1/1000) was used as the secondary antibody

    See Abreview

参考文献

This product has been referenced in:
  • Grois L  et al. Combined Inhibition of the Renin-Angiotensin System and Neprilysin Positively Influences Complex Mitochondrial Adaptations in Progressive Experimental Heart Failure. PLoS One 12:e0169743 (2017). Read more (PubMed: 28076404) »
  • Kadir R  et al. ALFY-Controlled DVL3 Autophagy Regulates Wnt Signaling, Determining Human Brain Size. PLoS Genet 12:e1005919 (2016). Read more (PubMed: 27008544) »

See all 2 Publications for this product

レビューと Q&A

Thank you for your enquiry.

I am pleased to provide the CoC for these antibodies.

If you have any further questions, please do not hesitate to contact me.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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