製品の概要

  • 製品名Donkey Anti-Rabbit IgG H&L (HRP)
    IgG 二次抗体 製品一覧
  • ターゲット生物種Rabbit
  • 特異性Reacts with the heavy and light chains of the immunoglobulin molecule.
  • アプリケーション適用あり: WBmore details
  • 免疫原

    Synthetic peptide corresponding to IgG.

  • 標識HRP

法規制情報

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C.
  • バッファーPreservative: 0.01% Thimerosal (merthiolate)
    Constituents: 50% Glycerol, 10mg/ml BSA
  • Concentration information loading...
  • 精製度Protein G purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 特記事項Part of the AbExcel range.
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab16284 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000 - 1/20000. Optimized for ECL applications.

Donkey Anti-Rabbit IgG H&L (HRP) 画像

  • All lanes : Anti-beta Actin antibody - Loading Control (ab8227) at 1 µg/ml

    Lanes 1-6 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lysates/proteins at 10 µg per lane.

    Secondary
    Lanes 1 - 2 : Donkey polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab16284) at 1/2000 dilution
    Lanes 3 - 4 : Donkey polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab16284) at 1/10000 dilution
    Lanes 5 - 6 : Donkey polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab16284) at 1/20000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Exposure time : 150 seconds
  • Ab16284 was used at dilution 1/5000 with the primary antibody ab52903 in ELISA. See the review on ab52903.
  • Ab16284 was used at dilution 1/2000 with the primary antibody ab28379 in WB. See the review on ab28379.

Donkey Anti-Rabbit IgG H&L (HRP) (ab16284) 使用論文

This product has been referenced in:
  • Mostyn A  et al. UCP1 is present in porcine adipose tissue and is responsive to postnatal leptin. J Endocrinol 223:M31-8 (2014). Read more (PubMed: 25122002) »
  • Fazeli AS  et al. Proteome analysis of post-transplantation recovery mechanisms of an EAE model of multiple sclerosis treated with embryonic stem cell-derived neural precursors. J Proteomics 94:437-50 (2013). Read more (PubMed: 23791935) »

See all 16 Publications for this product

Product Wall

We have issued a free of charge replacement.

Thank you for your reply. I recommend the following HRP conjugated secondary antibodies to use with these rabbit Anti-GAD65+GAD67 primary antibodies. I have included one product from a donkey host and another from goat. Both are guaranteed and the c...

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I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacements for one vial of ab63913 and one vial of ab16284 with the order number 1147505.
...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"