Donkey Anti-Rabbit IgG H&L (FITC) preadsorbed (ab97084)

製品の概要

  • 製品名Donkey Anti-Rabbit IgG H&L (FITC) preadsorbed
    IgG 二次抗体 製品一覧
  • 製品の詳細
    Donkey polyclonal Secondary Antibody to Rabbit IgG - H&L (FITC), pre-adsorbed
  • ターゲット生物種Rabbit
  • 特異性By immunoelectrophoresis and ELISA this antibody reacts specifically with Rabbit IgG and with light chains common to other Rabbit immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Less than 0.1% cross reactivity to bovine, chicken, goat, horse, human, mouse, pig and rat was detected.
  • アプリケーション適用あり: ICC/IF, IHC-P, Flow Cytmore details
  • 吸着処理血清
    Chicken, Cow, Goat, Human, Mouse, Pig, Rat more details
  • 標識FITC. Ex: 493nm, Em: 528nm

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C.
  • バッファーPreservative: 0.09% Sodium Azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)Antiserum was cross adsorbed using bovine, chicken, goat, human, mouse, pig and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to FITC.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab97084 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/50 - 1/500.
IHC-P 1/50 - 1/500.
Flow Cyt 1/50 - 1/200.

Donkey Anti-Rabbit IgG H&L (FITC) preadsorbed (ab97084) 使用論文

ab97084 has not yet been referenced specifically in any publications.

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Thank you very much for contacting us.

Please find attached the QC ICC/IF image for this antibody. The cell line is A172 cells. The antibodies used is at 5ug//ml and detected using Alexa Fluor 488 conjugated secondary antibody. The nuclei are ...

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We used the following protocol with Anti-Glucose Transporter GLUT4 antibody (ab62375):

NSO cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat...

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Thank you for contacting us in regards to pre-incubating a primary and secondary together prior to performing FACS analysis.

I have spoken to my colleague who is quite experienced in using flow cytometry and she has informed me that whilst pr...

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Thank you for contacting us.
I am sorry to hear that you received so far no staining with the antibodies.

As we discussed by phone, I'd suggest trying the following:

1) try another antigen retrieval step (e.g. Tris/EDTA, pH 8) ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"