Donkey Anti-Rabbit IgG H&L (DyLight® 650) preadsorbed (ab96922)
Key features and details
- Donkey Anti-Rabbit IgG H&L (DyLight® 650) preadsorbed
- Conjugation: DyLight® 650. Ex: 654nm, Em: 673nm
- Host species: Donkey
- Isotype: IgG
- Suitable for: ICC/IF, IHC-P, Flow Cyt
Related conjugates and formulations
製品の概要
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製品名
Donkey Anti-Rabbit IgG H&L (DyLight® 650) preadsorbed
IgG 二次抗体 製品一覧 -
由来種
Donkey -
ターゲット生物種
Rabbit -
特異性
By immunoelectrophoresis and ELISA this antibody reacts specifically with Rabbit IgG and with light chains common to other Rabbit immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Reduced cross-reactivity to bovine, chicken, goat, horse, human, mouse, pig and rat was detected.
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アプリケーション
適用あり: ICC/IF, IHC-P, Flow Cytmore details -
吸着処理血清
Chicken, Cow, Goat, Horse, Human, Mouse, Pig, Rat more details -
標識
DyLight® 650. Ex: 654nm, Em: 673nm
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.2% BSA, PBS -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
Antiserum was cross adsorbed using bovine, chicken, goat, human, mouse, pig and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 650. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
特記事項
DyLight® 650 replaces DyLight® 649. -
研究分野
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab96922の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF |
1/50 - 1/500.
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IHC-P |
1/50 - 1/500.
DyLight® 650 replaces DyLight® 649. The DyLight® 650 excitation is 652nm and the emission is 672nm. DyLight® 650 hence provides the same far-red fluorescence and photostability as the DyLight® 649 dye. Please refer to the vial in order to check whether the product you received is DyLight® 649 or DyLight® 650. This information however will have no impact on your experiments. For more information please refer to DyLight® 650. |
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Flow Cyt |
1/50 - 1/200.
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特記事項 |
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ICC/IF
1/50 - 1/500. |
IHC-P
1/50 - 1/500. DyLight® 650 replaces DyLight® 649. The DyLight® 650 excitation is 652nm and the emission is 672nm. DyLight® 650 hence provides the same far-red fluorescence and photostability as the DyLight® 649 dye. Please refer to the vial in order to check whether the product you received is DyLight® 649 or DyLight® 650. This information however will have no impact on your experiments. For more information please refer to DyLight® 650. |
Flow Cyt
1/50 - 1/200. |
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プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (13)
ab96922 は 13 報の論文で使用されています。
- Hamade H et al. BATF3 Protects Against Metabolic Syndrome and Maintains Intestinal Epithelial Homeostasis. Front Immunol 13:841065 (2022). PubMed: 35812447
- Basílio J et al. Antagonistic Functions of Androgen Receptor and NF-κB in Prostate Cancer-Experimental and Computational Analyses. Cancers (Basel) 14:N/A (2022). PubMed: 36551650
- Moser B et al. The inflammatory kinase IKKa phosphorylates and stabilizes c-Myc and enhances its activity. Mol Cancer 20:16 (2021). PubMed: 33461590
- Neddens J et al. Correlation of pyroglutamate amyloid ß and ptau Ser202/Thr205 levels in Alzheimer's disease and related murine models. PLoS One 15:e0235543 (2020). PubMed: 32645028
- Tan EE et al. Dominant-negative NFKBIA mutation promotes IL-1ß production causing hepatic disease with severe immunodeficiency. J Clin Invest 130:5817-5832 (2020). PubMed: 32750042