Donkey Anti-Rabbit IgG H&L (DyLight® 550) preadsorbed (ab96920)

製品の概要

  • 製品名Donkey Anti-Rabbit IgG H&L (DyLight® 550) preadsorbed
    IgG 二次抗体 製品一覧
  • 製品の詳細
    Donkey polyclonal Secondary Antibody to Rabbit IgG - H&L (DyLight® 550), pre-adsorbed
  • ターゲット生物種Rabbit
  • 特異性By immunoelectrophoresis and ELISA this antibody reacts specifically with Rabbit IgG and with light chains common to other Rabbit immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Less than 0.1% cross reactivity to bovine, chicken, goat, horse, human, mouse, pig and rat was detected.
  • アプリケーション適用あり: IHC-P, ICC/IF, Flow Cytmore details
  • 吸着処理血清
    Chicken, Cow, Goat, Horse, Human, Mouse, Pig, Rat more details
  • 標識DyLight® 550. Ex: 562nm, Em: 576nm

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C.
  • バッファーPreservative: 0.09% Sodium Azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)Antiserum was cross adsorbed using bovine, chicken, goat, human, mouse, pig and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 550.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 特記事項DyLight® 550 replaces DyLight® 549.
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab96920 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/50 - 1/500. DyLight®550 replaces DyLight®549. The DyLight®550 excitation is 562nm and the emission is 576nm. DyLight®550 provides the same orange-to-red fluorescence and photostability as the DyLight®549 dye. Please refer to the vial in order to check whether the product you received is DyLight®549 or DyLight®550. This information however will have no impact on your experiments. For more information please refer to www.abcam.com/DyLight550.
ICC/IF 1/50 - 1/500.
Flow Cyt 1/50 - 1/200.

Donkey Anti-Rabbit IgG H&L (DyLight® 550) preadsorbed 画像

  • Emission spectra of DyLight® fluorochromes available in our catalog.
    Line colors represent the approximate visible colors of the wavelength maxima.

Donkey Anti-Rabbit IgG H&L (DyLight® 550) preadsorbed (ab96920) 使用論文

ab96920 has not yet been referenced specifically in any publications.

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Thank you for your inquiry.

I strongly suggest to use a pre-absorbed secondary antibody so that the cross-binding to the two rat monoclonals can be minimized.

Since you are already using fluorescence in the green and red spectrum,...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"