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The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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7 reviews or Q&A
We have a flourocene filter, which is why I picked the original antibody.
Asked on Apr 26 2012
Thank you for your reply. I googled for flourocene filter, but could not find an entry. Do you maybe mean fluorescence filter? What wavelength does the filter cover? I assume it would be in the green range(about 480 - 570 nm) since...
Abcam Scientific Support
Answered on Apr 26 2012
1) Antibody is stored at -4 until use, just a normal freezer. When I use it I keep it in a refridgerator for no longer than 2 days. 2) Could you clarify this point? Are you saying I should not have added the PBS to the antibody vial? Only 50...
Asked on May 01 2012
Thank you for your email. To answer your questions and make some comments/suggestions that hopefully will help you further: 1) The antibodies (shipped as is) should be stored aliquoted and stored at -20 or -80 degree. This would als...
Answered on May 01 2012
The trypsin antigen retrieval did not work. I'd like to review what I did to see if there are any large errors. Rats wre perfused in situ and brains were preserved in 4% paraformaldehyde solution overnight. We purchased the paraformaldehyde ...
Asked on Apr 30 2012
Thank you for your email. I am sorry to hear the antigen retrieval step did not improve your results. I have looked throught your protocol you sent me and have the following suggestions, comments and questions: 1) You are makin...
Answered on Apr 30 2012
Thank you for the help today. I have already purchased ab970 (trypsin enzymatic antigen retrieval) after our conversation and will let you know how that works. I took 5 ul of secondary and it did flouresce so the antibody is not dead. I will try antige...
Asked on Apr 25 2012
Thank you for your reply. I'm glad to hear that the secondary antibody shows fluorescence. As for selecting another secondary antibody, could you please let me know which fluorophore or wavelength range is covered by your miscroscope? Ba...
Answered on Apr 25 2012
IHC-Fr with adult rat brain (hippocampus) no signal at all 20 um sections fixation: PFA 4% overnight Ab: ab6994: 1/200, 1/500, 1/800 o/n 4 degree ab104225: 1/500, 1/1000, 1/2000 o/n 4 degree block: none secondary: 1/2...
Thank you for contacting us. I am sorry to hear that the antibodie might not be working as expected. As we discussed over the phone the following troubleshooting tips might help in finding the cause of the problem: 1) Try antig...
COULD NOT EXPECT THAT YOU ARE STILL AT WORK, THOUGHT ONLY GUYS IN THE UNIVERSITIES STAY LATE... THANK YOU FOR YOUR HELP!!!! SINCERELY
Asked on Jan 13 2012
It has been a pleasure to help. We are here until 8:00pm eastern time so not quite as late as the university crowd. Please feel free to contact me if you have any questions.
Answered on Jan 13 2012
HI I WILL USE IT PRIMARILY FOR FLOW, BUT PLAN TO USE IT FOR IHC AS WELL THANK YOU
Asked on Jan 12 2012
Thank you for contacting Abcam. We have a large number of secondary antibodies with fluorescent tags. Theeasiest way to find the one that best fits your needs is from the Abcam home page. The secondary antibodies drop down, located in the yellow banner...
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