Donkey Anti-Rabbit IgG H&L (Alkaline Phosphatase) preadsorbed (ab7084)

Submit an Abreview Q&A (2)Specific References (1)

製品の概要

  • 製品名
    Donkey Anti-Rabbit IgG H&L (Alkaline Phosphatase) preadsorbed
    IgG 二次抗体 製品一覧
  • 由来種
    Donkey
  • ターゲット生物種
    Rabbit
  • アプリケーション
    適用あり: ICC/IF, IHC-P, WB, ELISA, Dot blot, IHC-Frmore details
  • 吸着処理血清

    Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Human, Mouse, Rat, Sheep more details
  • 免疫原

    Rabbit IgG whole molecule

  • 標識
    Alkaline Phosphatase

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    pH: 7.60
    Preservative: 0.01% Sodium azide
    Constituents: 0.88% Sodium chloride, 1% BSA, 50% Glycerol, 0.0014% Zinc chloride, 0.0095% Magnesium chloride, 0.79% Tris HCl
  • Concentration information loading...
  • 精製度
    Affinity purified
  • 特記事項（精製）
    Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons)
  • 特記事項（標識）
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities
  • ポリ/モノ
    ポリクローナル
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab7084 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/400 - 1/2000.
IHC-P Use at an assay dependent concentration.
WB 1/1000 - 1/5000.
ELISA 1/4000 - 1/16000.
Dot blot Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.

参考文献

This product has been referenced in:
  • Peng EW  et al. Myocardial expression of heat shock protein 70i protects early postoperative right ventricular function in cyanotic tetralogy of Fallot. J Thorac Cardiovasc Surg 141:1184-91 (2011). Read more (PubMed: 21397265) »

See 1 Publication for this product

ab7084 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

レビューと Q&A

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2 reviews or Q&A

10
Votes: Highest First

ich bin an einer immunohistochemischen Färbung von humanem CD8 und humanem FoxP3 (in Mausgewebe) interessiert.



- Idealerweise mit einen rabbit anti CD-8 Primärantikörper

- Und FoxP3 mit Alkalischer Phosp...

Read More

Vielen Dank für Ihre Anfrage.

Ich habe Ihnen hier vier Antikörper herausgesucht, die wenig bzw. gar keine Kreuzreaktion mit dem Maus-Protein aufweisen, so dass Sie nur das humane Protein im Mausgewebe detektieren sollten.

Drei der...

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Customer would like to know the crossreactivity of these two antibodies with human IgG? The datasheet states taht there is a minimum cross-reaction. Can you provide some data? % of cross-reaction? He is looking for a secondary antibody which does not c...

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Thank you for your phone call regarding cross-reactivity of ab7084 and ab7062. We have just received some more information and would like to pass it onto you: Our QC criteria are less than 1% cross-reactivity to those minimized serum proteins wh...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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