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Product Name
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Donkey polyclonal Secondary Antibody to Goat IgG - H&L (Cy5 ®), pre-adsorbed
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Product type
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Secondary antibodies
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Description
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Donkey polyclonal Secondary Antibody to Goat IgG - H&L (Cy5 ®), pre-adsorbed
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Immunogen
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Goat IgG, whole molecule
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Tested applications
(see key)
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Flow Cyt, ICC/IF, IHC-Fr, IHC-P
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Application notes
(see key)
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Recommended dilutions
Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Please refer to the relevant conjugation for dilutions specific for this product:
Conjugate |
ELISA |
Blotting |
Flow Cytometry |
IHC |
Fluorescein |
nd |
nd |
50 - 200 |
200 - 1,000 |
Rhodamine, Texas RedTM |
nd |
nd |
50 - 200 |
200 - 1,000 |
R-Phycoerythrin |
nd |
nd |
100 - 200 |
nd |
Cyanines |
nd |
nd |
400 |
100 - 1,000 |
Biotin |
5,000 - 20,000 |
3,000 - 10,000 |
nd |
100 - 1,000 |
Peroxidase |
4,000 - 10,000 |
2,000 - 5,000 |
nd |
200 - 2,000 |
Alkaline Phosphatase |
1,000 - 2,000 |
500 - 1,000 |
nd |
100 - 1,000 |
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Research areas
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Secondary antibodies >> anti-Goat >> IgG >> Fluorophore >> Cy5
Immunology >> Immunoglobulins >> Heavy Chain >> IgG
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Donkey polyclonal Secondary Antibody to Goat IgG - H&L (Cy5 ®), pre-adsorbed (ab6566)の別名 |
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Raised in
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Donkey
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Clonality
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Polyclonal
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Purity
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Immunogen affinity purified
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Conjugation notes
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Cy5.29 (Cyanine 5.29-OSu) (Molecular Weight 975 daltons)
Absorption Wavelength: 650 nm
Emission Wavelength: 667 nm
Fluorochrome/Protein Ratio: 6.0 moles Cy5 per mole of Goat IgG
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Storage buffer
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0.01M Na phosphate, 0.25M NaCl, pH7.6, 0.05% Na azide, 0.01% Thimerisol, 15mg/ml BSA Material safety datasheet (MSDS) for this product:
Sodium Phosphate MSDS
この製品は、毒物及び劇物取締法に基づく「毒物」に該当します。
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Purification notes
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This product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities and extensive dialysis.
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Form
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Liquid
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Conjugation
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Cy5 ®
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Notes
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This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5,268,486 and related patents. Cy and CyDye are trademarks of GE Healthcare Limited.
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Abcam では全ての製品情報を一元管理しており、 Donkey polyclonal Secondary Antibody to Goat IgG - H&L (Cy5 ®), pre-adsorbed について私たちが知りうる全ての情報がこのデータシートでご覧いただけます。ご不明な点がありましたら、 お問い合わせください。 |
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References for Donkey polyclonal Secondary Antibody to Goat IgG - H&L (Cy5 ®), pre-adsorbed (ab6566)
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This product has been used in: (two most recent references)
Cichon S et al. Brain-specific tryptophan hydroxylase 2 (TPH2): a functional Pro206Ser substitution and variation in the 5'-region are associated with bipolar affective disorder. Hum Mol Genet 17:87-97 (2008). PubMed: 17905754
Abdel Nour AM et al. Folate receptor and human reduced folate carrier expression in HepG2 cell line exposed to fumonisin B1 and folate deficiency. Carcinogenesis 28:2291-7 (2007). PubMed: 17615259
See all 3 publication references for this product.
If you publish research using ab6566 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to IgG
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Donkey polyclonal Secondary Antibody to Goat IgG - H&L (Cy5 ®), pre-adsorbed - more information
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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