Donkey Anti-Mouse IgG H&L (Phycoerythrin) preadsorbed (ab7003)

製品の概要

  • 製品名
    Donkey Anti-Mouse IgG H&L (Phycoerythrin) preadsorbed
    IgG 二次抗体 製品一覧
  • ターゲット生物種
    Mouse
  • アプリケーション
    適用あり: IHC-P, IHC-Fr, Immunomicroscopy, Flow Cyt, ICC/IFmore details
  • 吸着処理血清

    Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Human, Rabbit, Rat, Sheep more details
  • 免疫原

    Mouse IgG whole molecule

  • 標識
    Phycoerythrin. Ex: 488nm, Em: 575nm

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C.
  • バッファー
    Preservative: 0.01% Sodium Azide
    Constituents: 10mg/ml BSA, 0.15M Sodium chloride, 0.02M Potassium phosphate, pH 7.2
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    Prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation
  • 特記事項(標識)
    R-Phycoerythrin (R-PE) Molecular Weight 240,000 daltons) Absorption Wavelength: 488 nm Emission Wavelength: 575 nm OD566nm/OD280nm: 2.8
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 特記事項


    Can be used in immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.

  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab7003 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use at an assay dependent dilution.
IHC-Fr Use at an assay dependent dilution.
Immunomicroscopy Use at an assay dependent dilution.
Flow Cyt 1/100 - 1/250.
ICC/IF 1/100 - 1/250.

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  • Ab7003 was used with the primary antibody ab33315 in Flow Cyt. See Abreview on ab33315.

参考文献

This product has been referenced in:
  • Cheng W  et al. Role of the novel HSP90 inhibitor AUY922 in hepatocellular carcinoma: Potential for therapy. Mol Med Rep 12:2451-6 (2015). WB . Read more (PubMed: 25955495) »
  • Zhu J  et al. Effects of the novel heat shock protein 90 inhibitor AUY922 in renal cell carcinoma ACHN and 786-O cells. Oncol Lett 10:941-945 (2015). WB . Read more (PubMed: 26622600) »

See all 3 Publications for this product

レビューと Q&A

I’m sorry for the delay in getting back to you. I have discussed the problem the customer had been encountering with my colleague and as I mentioned the results they have been observing are what would be expected. The Alexa488 is a much stronger fluoro...

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Thank you for contacting us and sorry for the delay in getting back to you.
I have looked at the protocol your customer has been using and I would say that the results they have been observing are what would be expected. The Alexa488 is a much str...

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Thank you for contacting us.  Our F(ab')2 fragment secondary antibodies ab7002 and ab7003 will not contain the Fc region, and this will be covered under our Abpromise guarantee.  If any Fc reactivity is observed in your experiments, we would be happy t...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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