Donkey Anti-Mouse IgG H&L (DyLight® 550) (ab96876)

製品の概要

  • 製品名
    Donkey Anti-Mouse IgG H&L (DyLight® 550)
    IgG 二次抗体 製品一覧
  • ターゲット生物種
    Mouse
  • 特異性
    By immunoelectrophoresis and ELISA this antibody reacts specifically with Mouse IgG and with light chains common to other Mouse immunoglobulins. No antibody was detected against non immunoglobulin serum proteins.
  • アプリケーション
    適用あり: IHC-P, ICC/IF, Flow Cyt, WBmore details
  • 標識
    DyLight® 550. Ex: 562nm, Em: 576nm

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C.
  • バッファー
    Preservative: 0.09% Sodium Azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 550.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 特記事項
    DyLight® 550 replaces DyLight® 549.
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab96876 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/50 - 1/500. DyLight® 550 replaces DyLight®549. The DyLight® 550 excitation is 562nm and the emission is 576nm. DyLight®550 provides the same orange-to-red fluorescence and photostability as the DyLight®549 dye. Please refer to the vial in order to check whether the product you received is DyLight®549 or DyLight®550 – this information however will have no impact on your experiments. For more information please refer to www.abcam.com/DyLight550.
ICC/IF 1/50 - 1/500.
Flow Cyt 1/50 - 1/200.
WB Use at an assay dependent concentration.

画像

  • Emission spectra of DyLight® fluorochromes available in our catalog.
    Line colors represent the approximate visible colors of the wavelength maxima.
  • Courtesy of an anonymous Abreview

参考文献

This product has been referenced in:
  • de la Mare JA  et al. Extracellular Hsp90 and TGFß regulate adhesion, migration and anchorage independent growth in a paired colon cancer cell line model. BMC Cancer 17:202 (2017). Read more (PubMed: 28302086) »
  • Alarcan J  et al. Metabolism of the Marine Phycotoxin PTX-2 and Its Effects on Hepatic Xenobiotic Metabolism: Activation of Nuclear Receptors and Modulation of the Phase I Cytochrome P450. Toxins (Basel) 9:N/A (2017). IHC-P ; Human . Read more (PubMed: 28678150) »

See all 4 Publications for this product

レビューと Q&A

Thank you for contacting Abcam.

I managed to find antibodies against rat GFAP, FOX3, Iba1, and CD68 all raised in different hosts (chicken, rabbit, goat, and mouse, respectively). They each are highly rated by other researchers and have numero...

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Thank you for your enquiry.

ab96876 is a Donkey polyclonal anti mouse IgG secondary, and should therefore detect ab13579TRF2 antibody [4A794]which is a mouse IgG1 primary.

As stated on the datasheet, ab96876 has been tested in IHC...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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