Donkey Anti-Mouse IgG H&L (DyLight® 488) (ab96875)


  • 製品名Donkey Anti-Mouse IgG H&L (DyLight® 488)
    IgG 二次抗体 製品一覧
  • ターゲット生物種Mouse
  • 特異性By immunoelectrophoresis and ELISA this antibody reacts specifically with Mouse IgG and with light chains common to other Mouse immunoglobulins. No antibody was detected against non immunoglobulin serum proteins.
  • アプリケーション適用あり: ICC/IF, IHC-P, Flow Cytmore details
  • 免疫原

    Other Immunogen Type corresponding to Mouse IgG.

  • 標識DyLight® 488. Ex: 493nm, Em: 518nm


  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C.
  • バッファーPreservative: 0.09% Sodium Azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 488.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野


Our Abpromise guarantee covers the use of ab96875 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/50 - 1/500.
IHC-P 1/50 - 1/500.
Flow Cyt 1/50 - 1/200.

Donkey Anti-Mouse IgG H&L (DyLight® 488) 画像

  • ab7817 staining alpha smooth muscle actin in mouse primary colon myofibroblasts by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with acetone and blocked with 5% BSA for 30 minutes at 25°C. Samples were incubated with primary antibody (1/100 in PBS + 5% BSA) for 2 hour at 25°C. A DyLight® 488-conjugated donkey anti-mouse IgG H&L (ab96875) (1/1000) was used as the secondary antibody. Vimentin appears in red.

    See Abreview

  • Emission spectra of DyLight® fluorochromes available in our catalog.
    Line colors represent the approximate visible colors of the wavelength maxima.

Donkey Anti-Mouse IgG H&L (DyLight® 488) (ab96875) 使用論文

This product has been referenced in:
  • Polettini J  et al. Telomere Fragment Induced Amnion Cell Senescence: A Contributor to Parturition? PLoS One 10:e0137188 (2015). ICC/IF ; Human . Read more (PubMed: 26397719) »
  • Goggolidou P  et al. ATMIN is a transcriptional regulator of both lung morphogenesis and ciliogenesis. Development 141:3966-77 (2014). IF ; Mouse . Read more (PubMed: 25294941) »

See all 2 Publications for this product

Product Wall

Thank you for explaining this for me.

The antibodies mentioned, ab96875,ab96871,ab96877 andab96873 are all recommended to be used in a range of 1/50-1/500 for ICC and IF applications. This is only a starting point and optimisation of these co...

Read More

Thank you for contacting us and your interest in our products.

The volume of antibody required per experiment would depend on what experiment the customer is hoping to perform, and what volume of diluted antibody the customer usually used in ...

Read More

Thank you for your enquiry and interest.

You could certainly use our advanced search engine specifically designed for secondary antibodies to find the most suitable products for your studies:

Read More