Donkey Anti-Mouse IgG H&L (DyLight® 488) (ab96875)

製品の概要

  • 製品名Donkey Anti-Mouse IgG H&L (DyLight® 488)
    IgG 二次抗体 製品一覧
  • ターゲット生物種Mouse
  • 特異性By immunoelectrophoresis and ELISA this antibody reacts specifically with Mouse IgG and with light chains common to other Mouse immunoglobulins. No antibody was detected against non immunoglobulin serum proteins.
  • アプリケーション適用あり: ICC/IF, IHC-P, Flow Cytmore details
  • 免疫原

    Other Immunogen Type corresponding to Mouse IgG.

  • 標識DyLight® 488. Ex: 493nm, Em: 518nm

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C.
  • バッファーPreservative: 0.09% Sodium Azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 488.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab96875 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/50 - 1/500.
IHC-P 1/50 - 1/500.
Flow Cyt 1/50 - 1/200.

Donkey Anti-Mouse IgG H&L (DyLight® 488) 画像

  • ab7817 staining alpha smooth muscle actin in mouse primary colon myofibroblasts by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with acetone and blocked with 5% BSA for 30 minutes at 25°C. Samples were incubated with primary antibody (1/100 in PBS + 5% BSA) for 2 hour at 25°C. A DyLight® 488-conjugated donkey anti-mouse IgG H&L (ab96875) (1/1000) was used as the secondary antibody. Vimentin appears in red.

    See Abreview

  • Emission spectra of DyLight® fluorochromes available in our catalog.
    Line colors represent the approximate visible colors of the wavelength maxima.

Donkey Anti-Mouse IgG H&L (DyLight® 488) (ab96875) 使用論文

This product has been referenced in:
  • Polettini J  et al. Telomere Fragment Induced Amnion Cell Senescence: A Contributor to Parturition? PLoS One 10:e0137188 (2015). ICC/IF ; Human . Read more (PubMed: 26397719) »
  • Goggolidou P  et al. ATMIN is a transcriptional regulator of both lung morphogenesis and ciliogenesis. Development 141:3966-77 (2014). IF ; Mouse . Read more (PubMed: 25294941) »

See all 2 Publications for this product

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Thank you for explaining this for me.

The antibodies mentioned, ab96875,ab96871,ab96877 andab96873 are all recommended to be used in a range of 1/50-1/500 for ICC and IF applications. This is only a starting point and optimisation of these co...

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The volume of antibody required per experiment would depend on what experiment the customer is hoping to perform, and what volume of diluted antibody the customer usually used in ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"