Donkey Anti-Mouse IgG H&L (DyLight® 488) (ab96875)
Key features and details
- Donkey Anti-Mouse IgG H&L (DyLight® 488)
- Conjugation: DyLight® 488. Ex: 493nm, Em: 518nm
- Host species: Donkey
- Isotype: IgG
- Suitable for: ICC/IF, IHC-P, Flow Cyt
Related conjugates and formulations
製品の概要
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製品名
Donkey Anti-Mouse IgG H&L (DyLight® 488)
IgG 二次抗体 製品一覧 -
由来種
Donkey -
ターゲット生物種
Mouse -
特異性
By immunoelectrophoresis and ELISA this antibody reacts specifically with Mouse IgG and with light chains common to other Mouse immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. -
アプリケーション
適用あり: ICC/IF, IHC-P, Flow Cytmore details -
免疫原
Other Immunogen Type corresponding to Mouse IgG.
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標識
DyLight® 488. Ex: 493nm, Em: 518nm
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.2% BSA, PBS -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 488. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab96875の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
ICC/IF |
1/50 - 1/500.
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IHC-P |
1/50 - 1/500.
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Flow Cyt |
1/50 - 1/200.
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特記事項 |
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ICC/IF
1/50 - 1/500. |
IHC-P
1/50 - 1/500. |
Flow Cyt
1/50 - 1/200. |
画像
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ab7817 staining alpha smooth muscle actin in mouse primary colon myofibroblasts by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with acetone and blocked with 5% BSA for 30 minutes at 25°C. Samples were incubated with primary antibody (1/100 in PBS + 5% BSA) for 2 hour at 25°C. A DyLight® 488-conjugated donkey anti-mouse IgG H&L (ab96875) (1/1000) was used as the secondary antibody. Vimentin appears in red.
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Emission spectra of DyLight® fluorochromes available in our catalog.
Line colors represent the approximate visible colors of the wavelength maxima.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (18)
ab96875 は 18 報の論文で使用されています。
- Castellanos-Montiel MJ et al. An Optimized Workflow to Generate and Characterize iPSC-Derived Motor Neuron (MN) Spheroids. Cells 12:N/A (2023). PubMed: 36831212
- Jamalpoor A et al. A novel human stem cell-based biomarker assay for in vitro assessment of developmental toxicity. Birth Defects Res 114:1210-1228 (2022). PubMed: 35289129
- Chen CF et al. Characterization of Osteogenesis and Chondrogenesis of Human Decellularized Allogeneic Bone with Mesenchymal Stem Cells Derived from Bone Marrow, Adipose Tissue, and Wharton's Jelly. Int J Mol Sci 22:N/A (2021). PubMed: 34445692
- Dolgodilina E et al. Choroid plexus LAT2 and SNAT3 as partners in CSF amino acid homeostasis maintenance. Fluids Barriers CNS 17:17 (2020). PubMed: 32046769
- Jara C et al. Microencapsulation of cellular aggregates composed of differentiated insulin and glucagon-producing cells from human mesenchymal stem cells derived from adipose tissue. Diabetol Metab Syndr 12:66 (2020). PubMed: 32774470