Donkey Anti-Goat IgG H&L (DyLight® 550) preadsorbed (ab96936)
Key features and details
- Donkey Anti-Goat IgG H&L (DyLight® 550) preadsorbed
- Conjugation: DyLight® 550. Ex: 562nm, Em: 576nm
- Host species: Donkey
- Isotype: IgG
- Suitable for: IHC-P, ICC/IF, Flow Cyt
Related conjugates and formulations
製品の概要
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製品名
Donkey Anti-Goat IgG H&L (DyLight® 550) preadsorbed
IgG 二次抗体 製品一覧 -
由来種
Donkey -
ターゲット生物種
Goat -
特異性
By immunoelectrophoresis and ELISA this antibody reacts specifically with Goat IgG and with light chains common to other Goat immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Reduced cross-reactivity to chicken, horse, human, mouse, pig, rabbit and rat was detected.
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アプリケーション
適用あり: IHC-P, ICC/IF, Flow Cytmore details -
吸着処理血清
Chicken, Horse, Human, Mouse, Pig, Rabbit, Rat more details -
免疫原
Other Immunogen Type corresponding to Goat IgG.
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標識
DyLight® 550. Ex: 562nm, Em: 576nm
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.2% BSA, PBS -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
Antiserum was cross adsorbed using chicken, human, mouse, pig, rabbit and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 550. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
特記事項
DyLight® 550 replaces DyLight® 549. -
研究分野
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab96936の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
1/50 - 1/500.
DyLight® 550 replaces DyLight® 549. The DyLight® 550 excitation is 562nm and the emission is 576nm. DyLight® 550 provides the same orange-to-red fluorescence and photostability as the DyLight® 549 dye. Please refer to the vial in order to check whether the product you received is DyLight® 549 or DyLight® 550. This information however will have no impact on your experiments. For more information please refer to DyLight® 550. |
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ICC/IF |
1/50 - 1/500.
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Flow Cyt |
1/50 - 1/200.
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特記事項 |
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IHC-P
1/50 - 1/500. DyLight® 550 replaces DyLight® 549. The DyLight® 550 excitation is 562nm and the emission is 576nm. DyLight® 550 provides the same orange-to-red fluorescence and photostability as the DyLight® 549 dye. Please refer to the vial in order to check whether the product you received is DyLight® 549 or DyLight® 550. This information however will have no impact on your experiments. For more information please refer to DyLight® 550. |
ICC/IF
1/50 - 1/500. |
Flow Cyt
1/50 - 1/200. |
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プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (3)
ab96936 は 3 報の論文で使用されています。
- Neddens J et al. Correlation of pyroglutamate amyloid ß and ptau Ser202/Thr205 levels in Alzheimer's disease and related murine models. PLoS One 15:e0235543 (2020). PubMed: 32645028
- Miggitsch C et al. Human bone marrow adipocytes display distinct immune regulatory properties. EBioMedicine 46:387-398 (2019). PubMed: 31327694
- Cottle DL et al. c-MYC-induced sebaceous gland differentiation is controlled by an androgen receptor/p53 axis. Cell Rep 3:427-41 (2013). PubMed: 23403291