製品の概要

  • 製品名Donkey Anti-Goat IgG H&L (Biotin)
    IgG 二次抗体 製品一覧
  • ターゲット生物種Goat
  • アプリケーション適用あり: ICC/IF, Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, WBmore details
  • 免疫原

    Full length native Goat IgG (purified).

  • 標識Biotin

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 0.01% Sodium Azide
    Constituents: 10mg/ml BSA, 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2
  • Concentration information loading...
  • 精製度IgG fraction
  • 特記事項(精製)This product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • 特記事項(標識)Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per Rabbit IgG molecule
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab6884 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/1000 - 1/5000.
Dot blot Use at an assay dependent dilution.
ELISA 1/180000.
IHC-P Use at an assay dependent dilution.
IHC-Fr Use at an assay dependent dilution.
Immunomicroscopy Use at an assay dependent dilution.
WB 1/2000 - 1/10000.

Donkey Anti-Goat IgG H&L (Biotin) 画像

  • ab6884 used to stain samples of whole heads of embryonic day 15 (E15) mice by Immunohistochemistry (Formalin-fixed, paraffin-embedded sections).
    Comparison of four staining methods. The heads were flash-frozen directly in isopentane on dry-ice. Once hardened, they were embedded in O.C.T compound. Frozen sections were postfixed in 4% paraformaldehyde in PBS for 20 minutes and quenched in 1.5% hydrogen peroxide for 30 minutes. Sections were then blocked for 2 hours at room temperature with 5% donkey serum in Tris-buffered saline with 0.1% Triton-X100 and then incubated with a goat anti-Nurr1 primary antibody 1/200 in 1% donkey serum in TBS at 4°C overnight. Biotinylated donkey anti-goat (ab6884) 1/500 in 1% donkey serum in TBS was applied for 2 hours at room temperature and reacted with avidin-biotinylated enzyme complex (ABC) using the Vectastain Elite kit and diaminobenzidene (DAB) according to the manufacturer's instructions. Images were taken directly on the PALM Microbeam to demonstrate the staining and image quality available to identify the different cell populations.

    Image a: Hematoxylin.
    Image b: Hematoxylin and Eosin.
    Image c: 0.1% cresyl violet in H2O.
    Image d: 1% cresyl violet in H2O.
    For hematoxylin and H&E staining (a, b), sections were rinsed with 70% EtOH and H2O, stained with Mayer's hematoxylin for 15 seconds, rinsed with H2O and 70% EtOH and stained with Accustain Eosin Y for 10 seconds (for H&E only). For cresyl violet staining (c, d), sections were rehydrated in decreasing concentrations of ethanol, stained in cresyl violet for 45 seconds and dehydrated in increasing concentrations of ethanol. 1% cresyl violet (d) provided the best morphological details and allowed clear distinction between cortical plate (CP) and subplate (SP).

Donkey Anti-Goat IgG H&L (Biotin) (ab6884) 使用論文

This product has been referenced in:
  • Jung CS  et al. Endogenous nitric-oxide synthase inhibitor ADMA after acute brain injury. Int J Mol Sci 15:4088-103 (2014). IHC-Fr . Read more (PubMed: 24663083) »
  • Tan JY  et al. Cross-talking noncoding RNAs contribute to cell-specific neurodegeneration in SCA7. Nat Struct Mol Biol 21:955-61 (2014). WB . Read more (PubMed: 25306109) »

See all 7 Publications for this product

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The Donkey polyclonal Secondary Antibody to Goat IgG - H&L (Biotin) (ab6884) is biotin conjugated. This antibody has been shown to work successfully in Western blotting and ca...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"