Donkey Anti-Goat IgG H&L (Biotin) (ab208000)
Key features and details
- Donkey Anti-Goat IgG H&L (Biotin)
- Conjugation: Biotin
- Host species: Donkey
- Isotype: IgG
- Suitable for: WB, Flow Cyt, ICC/IF, IHC-Fr, IP, ELISA, IHC-P
Related conjugates and formulations
製品の概要
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製品名
Donkey Anti-Goat IgG H&L (Biotin)
IgG 二次抗体 製品一覧 -
由来種
Donkey -
ターゲット生物種
Goat -
アプリケーション
適用あり: WB, Flow Cyt, ICC/IF, IHC-Fr, IP, ELISA, IHC-Pmore details -
免疫原
Full length protein corresponding to Goat IgG.
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標識
Biotin
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA, 30% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Affinity purified -
特記事項(精製)
Immunogen affinity purified - This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Biotin. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab208000の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use at an assay dependent concentration.
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Flow Cyt |
Use at an assay dependent concentration.
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ICC/IF |
Use at an assay dependent concentration.
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IHC-Fr |
Use at an assay dependent concentration.
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IP |
Use at an assay dependent concentration.
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ELISA |
1/20000 - 1/200000.
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IHC-P |
1/500 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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特記事項 |
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WB
Use at an assay dependent concentration. |
Flow Cyt
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. |
IHC-Fr
Use at an assay dependent concentration. |
IP
Use at an assay dependent concentration. |
ELISA
1/20000 - 1/200000. |
IHC-P
1/500 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
画像
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IHC image of alpha Tubulin staining in a section of formalin-fixed paraffin-embedded normal human colon tissue*. Ab208000 Donkey Anti-Goat IgG H & L (Biotin) was used as the secondary antibody.
Staining was performed on a Leica BondTM. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins, before blocking of endogenous biotin using ab64212. The section was then incubated with ab7291, 1/100 dilution, for 15 mins at room temperature. Ab98800 (Goat anti-Mouse IgG) was added as a bridging antibody at 1/250 dilution for 1 hour, followed by ab208000, 1/500 dilution, for 15 mins at room temperature. Detection was via an HRP conjugated ABC system and DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
The inset negative control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
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ab208000 was tested by direct ELISA, where wells were coated with serially diluted goat IgG (1000 – 16 ng/ml) for 2 hours, followed by a 2 hour blocking step (5% BSA). ab208000 (1:20,000 dilution; 2 hours) was added and detected by streptavidin-HRP (ab7403; 1:10,000 dilution; 1 hour). Signal was developed by TMB substrate. Data from duplicates; +/- SD.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab208000 は 3 報の論文で使用されています。
- Chen K et al. The beneficial effects of commensal E. coli for colon epithelial cell recovery are related with Formyl peptide receptor 2 (Fpr2) in epithelial cells. Gut Pathog 15:28 (2023). PubMed: 37322488
- Akiba K et al. POU1F1/Pou1f1 c.143-83A > G Variant Disrupts the Branch Site in Pre-mRNA and Leads to Dwarfism. Endocrinology 164:N/A (2022). PubMed: 36427334
- Zhao H et al. Long non-coding RNA SNHG17 enhances the aggressiveness of C4-2 human prostate cancer cells in association with ß-catenin signaling. Oncol Lett 21:472 (2021). PubMed: 33907582