Donkey Anti-Goat IgG H&L (Alkaline Phosphatase) (ab97107)

製品の概要

  • 製品名Donkey Anti-Goat IgG H&L (Alkaline Phosphatase)
    IgG 二次抗体 製品一覧
  • ターゲット生物種Goat
  • 特異性By immunoelectrophoresis and ELISA this antibody reacts specifically with Goat IgG and with light chains common to other Goat immunoglobulins. No antibody was detected against non immunoglobulin serum proteins.
  • アプリケーション適用あり: ICC, IHC-P, ELISA, WBmore details
  • 標識Alkaline Phosphatase

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C.
  • バッファーPreservative: 0.09% Sodium Azide
    Constituents: 0.2% BSA, 0.1M Sodium chloride, 50mM HEPES, 1mM Magnesium chloride, 0.1mM Zinc chloride, pH 7.1
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Alkaline Phosphatase (AP).
  • 特記事項(標識)Molar enzyme: antibody protein ratio is 1:1
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 特記事項Part of the AbExcel range.
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab97107 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC Use at an assay dependent dilution.
IHC-P 1/100 - 1/1000.
ELISA 1/5000 - 1/50000. (Primary).
WB 1/5000 - 1/50000. (Chemiluminescent).

Donkey Anti-Goat IgG H&L (Alkaline Phosphatase) 画像

  • All lanes : Anti-beta Tubulin antibody - Loading Control (ab21057) at 1 µg/ml and HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µg per lane.

    Secondary
    Lanes 1 - 2 : Donkey polyclonal Secondary Antibody to Goat IgG - H&L (AP) (ab97107) at 1/5000 dilution
    Lanes 3 - 4 : Donkey polyclonal Secondary Antibody to Goat IgG - H&L (AP) (ab97107) at 1/20000 dilution
    Lanes 5 - 6 : Donkey polyclonal Secondary Antibody to Goat IgG - H&L (AP) (ab97107) at 1/50000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Exposure time : 8 minutes

Donkey Anti-Goat IgG H&L (Alkaline Phosphatase) (ab97107) 使用論文

ab97107 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us with your question. For the alkaline phosphatase conjugated antibody ab97107, you can use the liquid fast-red kit ab64254- http://www.abcam.com/Liquid-Fast-Red-Substrate-Kit-ab64254.html For the HRP conjugated antib...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"