Donkey F(ab')2 Anti-Rat IgG - H&L (DyLight® 488), pre-adsorbed (ab102232)

製品の概要

  • 製品名Donkey F(ab')2 Anti-Rat IgG - H&L (DyLight® 488), pre-adsorbed
    IgG 二次抗体 製品一覧
  • ターゲット生物種Rat
  • 特異性By immunoelectrophoresis and ELISA this antibody reacts specifically with rat IgG and with light chains common to other rat immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to bovine, chicken, goat, horse, human, mouse, rabbit and sheep IgG was detected. This antibody may cross react with IgG from other species.
  • アプリケーション適用あり: IHC-P, ICC, Flow Cytmore details
  • 吸着処理血清
    Chicken, Cow, Goat, Horse, Human, Mouse, Rabbit, Sheep more details
  • 標識DyLight® 488. Ex: 493nm, Em: 518nm

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C.
  • バッファーPreservative: 0.09% Sodium Azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)Antiserum was solid phase absorbed to ensure class specificity. Antiserum was cross absorbed using bovine, chicken, goat, horse, human, mouse, rabbit and sheep immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragments were generated using a pepsin digestion. Fc fragments and whole IgG molecules have been removed. Fragments were conjugated to DyLight® 488.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab102232 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/50 - 1/500.
ICC 1/50 - 1/500.
Flow Cyt 1/50 - 1/200.

Donkey F(ab')2 Anti-Rat IgG - H&L (DyLight® 488), pre-adsorbed 画像

  • Sample: mouse embryonic stem cell-differentiated embryoid bodies (EBs)

    Preparation:

    • Fix in 3%PFA in PBS for 30 min at RT
    • Incubate in 7.5% sucrose-PBS for 3h at RT
    • Incubate in 15% sucrose-PBS at 4 degree Celsius overnight
    • Embed the EBs in tissue-Tek OCT compound
    • Cut frozen sections to 4-20 µm thickness


    Primary antibody: Rat anti-perlecan (basement membrane marker), 1:100
    Secondary antibody: Donkey F(ab')2 polyclonal to Rat IgG - H&L DyLight® 488 (ab102232), 1:200
    Nuclei were counterstained with DAPI

Donkey F(ab')2 Anti-Rat IgG - H&L (DyLight® 488), pre-adsorbed (ab102232) 使用論文

This product has been referenced in:
  • Xia F  et al. IRGM1 regulates oxidized LDL uptake by macrophage via actin-dependent receptor internalization during atherosclerosis. Sci Rep 3:1867 (2013). Read more (PubMed: 23689639) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"