Donkey F(ab')2 Anti-Mouse IgG - H&L (DyLight® 650), pre-adsorbed (ab98769)

製品の概要

  • 製品名
    Donkey F(ab')2 Anti-Mouse IgG - H&L (DyLight® 650), pre-adsorbed
    IgG 二次抗体 製品一覧
  • ターゲット生物種
    Mouse
  • アプリケーション
    適用あり: Flow Cyt, ICC/IF, IHC-Pmore details
  • 吸着処理血清

    Chicken, Cow, Goat, Horse, Human, Rabbit, Rat, Sheep more details
  • 標識
    DyLight® 650. Ex: 654nm, Em: 673nm

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C.
  • バッファー
    Preservative: 0.09% Sodium Azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    This antibody was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragment were generated using a pepsin digestion. Fc fragments and whole IgG molecules have been removed. Fragments were conjugated to DyLight® 650.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 特記事項
    DyLight® 650 replaces DyLight® 649.
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab98769 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt 1/50 - 1/200.
ICC/IF 1/50 - 1/500.
IHC-P 1/50 - 1/500. DyLight®650 replaces DyLight®649. The DyLight®650 excitation is 652nm and the emission is 672nm. DyLight®650 hence provides the same far-red fluorescence and photostability as the DyLight®649 dye. Please refer to the vial in order to check whether the product you received is DyLight®649 or DyLight®650. This information however will have no impact on your experiments. For more information please refer to www.abcam.com/DyLight650.

画像

  • ab18005 staining phosphatidylserine in rat carotid artery tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was permeabilized with 50% ethanol in TPBS and blocked with 15% normal horse serum for 1 hour at room temperature. Samples were incubated with primary antibody (1/100 in 15% normal horse serum) for 18 hours at 4°C. A DyLight® 650-conjugated Donkey F(ab')2 anti-mouse IgG H&L (ab98769) (1/500) was used as the secondary antibody.

    See Abreview

  • Emission spectra of DyLight® fluorochromes available in our catalog.
    Line colors represent the approximate visible colors of the wavelength maxima.

参考文献

This product has been referenced in:
  • Qian H  et al. Molecular characterization of prospectively isolated multipotent mesenchymal progenitors provides new insight into the cellular identity of mesenchymal stem cells in mouse bone marrow. Mol Cell Biol 33:661-77 (2013). IHC-Fr . Read more (PubMed: 23184664) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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