Donkey F(ab')2 Anti-Mouse IgG - H&L (DyLight® 488), pre-adsorbed (ab98766)

製品の概要

  • 製品名
    Donkey F(ab')2 Anti-Mouse IgG - H&L (DyLight® 488), pre-adsorbed
    IgG 二次抗体 製品一覧
  • ターゲット生物種
    Mouse
  • アプリケーション
    適用あり: Flow Cyt, ICC/IF, IHC-Pmore details
  • 吸着処理血清

    Chicken, Cow, Goat, Horse, Human, Rabbit, Rat, Sheep more details
  • 標識
    DyLight® 488. Ex: 493nm, Em: 518nm

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C.
  • バッファー
    Preservative: 0.09% Sodium Azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    This antibody was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragment were generated using a pepsin digestion. Fc fragments and whole IgG molecules have been removed. Fragments were conjugated to DyLight® 488.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab98766 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt 1/50 - 1/200.
ICC/IF 1/50 - 1/500.
IHC-P 1/50 - 1/500.

画像

  • Sample: mouse embryonic stem cell-differentiated embryoid bodies (EBs)

    Preparation:

    • Fix in 3%PFA in PBS for 30 min at RT
    • Incubate in 7.5% sucrose-PBS for 3h at RT
    • Incubate in 15% sucrose-PBS at 4 degree Celsius overnight
    • Embed the EBs in tissue-Tek OCT compound
    • Cut frozen sections to 4-20 µm thickness


    Primary antibodies:
    Mouse anti-disabled-2 (endoderm marker), 1:100
    Rabbit anti-lamin a 1 (basement membrane marker), 1:400

    Secondary antibodies:
    Donkey F(ab')2 polyclonal Secondary Antibody to Mouse IgG -H&L (DyLight® 488), pre-adsorbed (ab98766), 1:200
    Goat anti-rabbit IgG Cy3®, 1:200

    Nuclei were counterstained with DAPI
  • Emission spectra of DyLight® fluorochromes available in our catalog.
    Line colors represent the approximate visible colors of the wavelength maxima.

参考文献

This product has been referenced in:
  • Khan MA  et al. JNK Activation Turns on LPS- and Gram-Negative Bacteria-Induced NADPH Oxidase-Dependent Suicidal NETosis. Sci Rep 7:3409 (2017). IHC-P ; Escherichia coli . Read more (PubMed: 28611461) »

See 1 Publication for this product

レビューと Q&A

Thank you for your enquiry.

I can confirm it is not unusual for an antibody to be kept at 4oC. The datasheet for many of our antibodies recommend to store at this temperature. At this temperature, they should be stable for at least 1 year. (...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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