Donkey F(ab')2 Anti-Mouse IgG - H&L (DyLight® 488), pre-adsorbed (ab98766)

製品の概要

  • 製品名Donkey F(ab')2 Anti-Mouse IgG - H&L (DyLight® 488), pre-adsorbed
    IgG 二次抗体 製品一覧
  • ターゲット生物種Mouse
  • アプリケーション適用あり: Flow Cyt, ICC/IF, IHC-Pmore details
  • 吸着処理血清
    Chicken, Cow, Goat, Horse, Human, Rabbit, Rat, Sheep more details
  • 標識DyLight® 488. Ex: 493nm, Em: 518nm

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C.
  • バッファーPreservative: 0.09% Sodium Azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)This antibody was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragment were generated using a pepsin digestion. Fc fragments and whole IgG molecules have been removed. Fragments were conjugated to DyLight® 488.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab98766 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt 1/50 - 1/200.
ICC/IF 1/50 - 1/500.
IHC-P 1/50 - 1/500.

Donkey F(ab')2 Anti-Mouse IgG - H&L (DyLight® 488), pre-adsorbed 画像

  • Sample: mouse embryonic stem cell-differentiated embryoid bodies (EBs)

    Preparation:

    • Fix in 3%PFA in PBS for 30 min at RT
    • Incubate in 7.5% sucrose-PBS for 3h at RT
    • Incubate in 15% sucrose-PBS at 4 degree Celsius overnight
    • Embed the EBs in tissue-Tek OCT compound
    • Cut frozen sections to 4-20 µm thickness


    Primary antibodies:
    Mouse anti-disabled-2 (endoderm marker), 1:100
    Rabbit anti-lamin a 1 (basement membrane marker), 1:400

    Secondary antibodies:
    Donkey F(ab')2 polyclonal Secondary Antibody to Mouse IgG -H&L (DyLight® 488), pre-adsorbed (ab98766), 1:200
    Goat anti-rabbit IgG Cy3®, 1:200

    Nuclei were counterstained with DAPI
  • Emission spectra of DyLight® fluorochromes available in our catalog.
    Line colors represent the approximate visible colors of the wavelength maxima.

Donkey F(ab')2 Anti-Mouse IgG - H&L (DyLight® 488), pre-adsorbed (ab98766) 使用論文

ab98766 has not yet been referenced specifically in any publications.

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I can confirm it is not unusual for an antibody to be kept at 4oC. The datasheet for many of our antibodies recommend to store at this temperature. At this temperature, they should be stable for at least 1 year. (...

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