Donkey F(ab')2 Anti-Human IgG - H&L (PE), pre-adsorbed (ab7005)

製品の概要

  • 製品名Donkey F(ab')2 Anti-Human IgG - H&L (PE), pre-adsorbed
    IgG 二次抗体 製品一覧
  • ターゲット生物種Human
  • アプリケーション適用あり: ICC/IF, IHC-P, IHC-Fr, Immunomicroscopy, Flow Cytmore details
  • 吸着処理血清
    Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Mouse, Rabbit, Rat, Sheep more details
  • 免疫原

    Human IgG whole molecule

  • 標識Phycoerythrin. Ex: 488nm, Em: 575nm

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C.
  • バッファーpH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 0.878% Sodium chloride, 1% BSA, 0.424% Potassium phosphate
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)Prepared from monospecific antiserum by immunoaffinity chromatography using human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation
  • 特記事項(標識)R-Phycoerythrin (R-PE) Molecular Weight 240,000 daltons) Absorption Wavelength: 488 nm Emission Wavelength: 575 nm OD566nm/OD280nm: 2.8
  • ポリ/モノポリクローナル
  • 特記事項


    Can be used in immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.

  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab7005 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/100 - 1/250.
IHC-P 1/50 - 1/200.
IHC-Fr 1/50 - 1/200.
Immunomicroscopy 1/50 - 1/200.
Flow Cyt 1/100 - 1/250.
The maximum amount of reagent required to stain 1 x 106 cells in flow cytometry is approximately 1.0 ug of antibody conjugate.
Lesser amounts of reagent may be sufficient for staining.

Donkey F(ab')2 Anti-Human IgG - H&L (PE), pre-adsorbed (ab7005) 使用論文

ab7005 has not yet been referenced specifically in any publications.

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I just received this information from the source of the antibody: 1. The immunogen of Human IgG whole molecule means the antibody recognizes any epitope on the whole human IgG, not just Fab portion. 2. "..absence of F(c) mediated binding" means...

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I just received this information from the source of the antibody: 1. The immunogen of Human IgG whole molecule means the antibody recognizes any epitope on the whole human IgG, not just Fab portion. 2. "..absence of F(c) mediated binding" means...

Read More

Thank you for your enquiry. The concentration of this product is 0.5 mg/mL. The total would be 500ug.

The Phycobiliprotein concentration of ab7005 is 0.5mg/mL. For both ab7005 and ab7006, there is one PE molecule with at least one molecule of active antibody. If you have any more questions, please contact us again.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"