Donkey F(ab')2 Anti-Human IgG - H&L (PE), pre-adsorbed (ab7005)
Key features and details
- Donkey F(ab')2 Anti-Human IgG - H&L (PE), pre-adsorbed
- Conjugation: PE. Ex: 488nm, Em: 575nm
- Host species: Donkey
- Suitable for: ICC/IF, IHC-P, IHC-Fr, Immunomicroscopy, Flow Cyt
製品の概要
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製品名
Donkey F(ab')2 Anti-Human IgG - H&L (PE), pre-adsorbed
IgG 二次抗体 製品一覧 -
由来種
Donkey -
ターゲット生物種
Human -
アプリケーション
適用あり: ICC/IF, IHC-P, IHC-Fr, Immunomicroscopy, Flow Cytmore details -
吸着処理血清
Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Mouse, Rabbit, Rat, Sheep more details -
免疫原
Human IgG whole molecule
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標識
PE. Ex: 488nm, Em: 575nm
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 6.5
Preservative: 0.01% Sodium azide
Constituents: 0.878% Sodium chloride, 1% BSA, 0.424% Tripotassium orthophosphate -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
Prepared from monospecific antiserum by immunoaffinity chromatography using human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation -
特記事項(標識)
R-Phycoerythrin (R-PE) Molecular Weight 240,000 daltons) Absorption Wavelength: 488 nm Emission Wavelength: 575 nm OD566nm/OD280nm: 2.8 -
ポリ/モノ
ポリクローナル -
特記事項
Can be used in immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
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研究分野
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab7005の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF |
1/100 - 1/250.
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IHC-P |
1/50 - 1/200.
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IHC-Fr |
1/50 - 1/200.
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Immunomicroscopy |
1/50 - 1/200.
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|
Flow Cyt |
1/100 - 1/250.
The maximum amount of reagent required to stain 1 x 106 cells in flow cytometry is approximately 1.0 ug of antibody conjugate.Lesser amounts of reagent may be sufficient for staining. |
特記事項 |
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ICC/IF
1/100 - 1/250. |
IHC-P
1/50 - 1/200. |
IHC-Fr
1/50 - 1/200. |
Immunomicroscopy
1/50 - 1/200. |
Flow Cyt
1/100 - 1/250. The maximum amount of reagent required to stain 1 x 106 cells in flow cytometry is approximately 1.0 ug of antibody conjugate. Lesser amounts of reagent may be sufficient for staining. |
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab7005 は 2 報の論文で使用されています。
- Avivi-Mintz S et al. Highly Sensitive and Specific SARS-CoV-2 Serological Assay Using a Magnetic Modulation Biosensing System. Biosensors (Basel) 12:N/A (2021). PubMed: 35049635
- Zhang Y et al. Anti-hypoglycemic and hepatocyte-protective effects of hyperoside from Zanthoxylum bungeanum leaves in mice with high-carbohydrate/high-fat diet and alloxan-induced diabetes. Int J Mol Med 41:77-86 (2018). PubMed: 29115390