Donkey F(ab')2 Anti-Human IgG - H&L (PE), pre-adsorbed (ab102439)

製品の概要

  • 製品名
    Donkey F(ab')2 Anti-Human IgG - H&L (PE), pre-adsorbed
    IgG 二次抗体 製品一覧
  • ターゲット生物種
    Human
  • 特異性
    By immunoelectrophoresis and ELISA this antibody reacts specifically with human IgG and with light chains common to other human immunoglobulins. Cross reactivity with IgA and IgM is negligible. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to bovine, chicken, goat, horse, mouse, rabbit, rat and sheep IgG was detected. This antibody may cross react with IgG from other species.
  • アプリケーション
    適用あり: Flow Cytmore details
  • 吸着処理血清

    Chicken, Cow, Goat, Horse, Mouse, Rabbit, Rat, Sheep more details
  • 標識
    Phycoerythrin. Ex: 488nm, Em: 575nm

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C.
  • バッファー
    Preservative: 0.09% Sodium Azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    Antiserum was cross adsorbed using bovine, chicken, goat, horse, mouse, rabbit, rat and sheep immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragments were generated using a pepsin digestion. Fc fragments and whole IgG molecules have been removed. Fragments were conjugated to Phycoerythrin.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab102439 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt 1/50 - 1/200.

Donkey F(ab')2 Anti-Human IgG - H&L (PE), pre-adsorbed (ab102439) 使用論文

ab102439 has not yet been referenced specifically in any publications.

Product Wall

Application
Flow Cytometry
CHO cell line expressing membrane bound human TNFα (stable transfectants) was incubated with 10μg/ml Remicade (anti-human TNFa monoclonal antibody) for 1 h in 4°C. The unbound antibody was washed off by centrifugation (300x g for 5min) and binding of remicade was detected with PE Donkey F(ab)2 anti-hIgG H&L (ab102439) – 1:100 (5ug/ml), 30 min incubation in 4°C.

PE donkey F(ab)2 anti-hIgG detected binding of remicade to TNFα CHO cell line giving strong positive signal and displaying minimal non-specific binding the cells alone, non-treated with remicade.
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投稿 Apr 25 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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