製品の概要

  • 製品名
    Donkey F(ab) Anti-Goat IgG H&L (Biotin)
    IgG 二次抗体 製品一覧
  • ターゲット生物種
    Goat
  • 特異性
    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin and anti-Donkey Serum. No reaction was observed against anti-Papain or anti-Donkey IgG F(c).
  • アプリケーション
    適用あり: ICC/IF, Dot blot, WB, ELISA, IHC, Immunomicroscopymore details
  • 免疫原

    Goat IgG whole molecule

  • 標識
    Biotin

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    Preservative: 0.01% Sodium Azide
    Constituents: 10mg/ml BSA (IgG and protease free), 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2
  • Concentration information loading...
  • 特記事項(精製)
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, papain digestion and chromatographic separation.
  • 特記事項(標識)
    Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per Donkey IgG Fab molecule
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab6578 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF
Dot blot
WB
ELISA
IHC
Immunomicroscopy
  • 追加情報
    Dot: Use at an assay dependent dilution.
    ELISA: 1/8000 - 1/36000.
    IHC: Use at an assay dependent dilution.
    IM: Use at an assay dependent dilution.
    WB: Use at an assay dependent dilution.

    This product has been assayed against 1.0 µg of Goat IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1/8000 to 1/36000 of the reconstitution concentration is suggested for this product.

    Suitable for other antibody based assays using streptavidin or avidin conjugates requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 参考文献

    This product has been referenced in:
    • Graham BB  et al. Schistosomiasis-induced experimental pulmonary hypertension: role of interleukin-13 signaling. Am J Pathol 177:1549-61 (2010). IHC-P . Read more (PubMed: 20671265) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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