製品の概要

  • 製品名Anti-DOK7 antibody
    DOK7 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to DOK7
  • アプリケーション適用あり: WB, IHC-P, Indirect ELISA, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Synthetic peptide derived from N-terminus of human DOK7.

  • ポジティブ・コントロール
    • human brain tissue, HepG2 cells, extracts from mouse brain cells.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab75049 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa).
IHC-P 1/50 - 1/100.
Indirect ELISA 1/5000.
ICC/IF 1/500 - 1/1000.

ターゲット情報

  • 機能Probable muscle-intrinsic activator of MUSK that plays an essential role in neuromuscular synaptogenesis. Acts in aneural activation of MUSK and subsequent acetylcholine receptor (AchR) clustering in myotubes. Induces autophosphorylation of MUSK.
  • 組織特異性Preferentially expressed in skeletal muscle and heart Present in thigh muscle, diaphragm and heart but not in the liver or spleen (at protein level).
  • 関連疾患Defects in DOK7 are the cause of familial limb-girdle myasthenia autosomal recessive (LGM) [MIM:254300]; also called congenital myasthenic syndrome type 1B or CMS1B. LGM is a congenital myasthenic syndrome characterized by a typical 'limb girdle' pattern of muscle weakness with small, simplified neuromuscular junctions but normal acetylcholine receptor and acetylcholinesterase function.
  • 配列類似性Contains 1 IRS-type PTB domain.
    Contains 1 PH domain.
  • 細胞内局在Cell membrane. Cell junction > synapse. Accumulates at neuromuscular junctions.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Docking protein 7 antibody
    • DOK 7 antibody
    • DOK7 antibody
    • DOK7_HUMAN antibody
    • Downstream of tyrosine kinase 7 antibody
    • Protein Dok-7 antibody
    see all

Anti-DOK7 antibody 画像

  • Human brain tissue with ab75049 at 1/50 dilution, in the absence and presence of the immunising peptide.
  • HepG2 cells with ab75049 at 1/500 dilution, in the absence and presence of the immunising peptide.
  • All lanes : Anti-DOK7 antibody (ab75049) at 1/500 dilution

    Lane 1 : extracts from mouse brain cells
    Lane 2 : extracts from mouse brain cells with immunising peptide at 5 µg

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 53 kDa
    Observed band size : 53 kDa
    Additional bands at : 46 kDa,80 kDa. We are unsure as to the identity of these extra bands.

Anti-DOK7 antibody (ab75049) 使用論文

ab75049 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Frozen sections)
Sample Rat Tissue sections (Tibialis anterior muscle)
Specification Tibialis anterior muscle
Fixative Paraformaldehyde
Permeabilization Yes - Triton X-100, 3% in TBS
Username

Mr. Alejandro Gomez

Verified customer

投稿 Sep 10 2012

DISCOUNT CODE: 000000


Expiration date: 05. October 2012

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