製品の概要

  • 製品名Anti-DOK2 antibody
    DOK2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to DOK2
  • 特異性Binds to a 55-60 kDa protein.
  • アプリケーション適用あり: IHC-P, WB, ELISA, ICCmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse
  • 免疫原

    20 amino acid C-terminal peptide to p56 dok2 RasGAP-binding protein

  • ポジティブ・コントロール
    • Human U937 cells or T-cell line Jurkatt.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab1677 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 2 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB 1/1000.
ELISA 1/100000.
ICC 1/300.

ターゲット情報

  • 機能DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK2 may modulate the cellular proliferation induced by IL-4, as well as IL-2 and IL-3. May be involved in modulating Bcr-Abl signaling. Attenuates EGF-stimulated MAP kinase activation.
  • 組織特異性Highly expressed in peripheral blood leukocytes, lymph nodes and spleen. Lower expression in thymus, bone marrow and fetal liver.
  • 配列類似性Belongs to the DOK family. Type A subfamily.
    Contains 1 IRS-type PTB domain.
    Contains 1 PH domain.
  • ドメインPTB domain mediates receptor interaction.
  • 翻訳後修飾On immunoreceptor stimulation, phosphorylated on C-terminal tyrosine residues. Phosphorylation on Tyr-345 is required for binding to the SH2 domain of NCK. Phosphorylation on both Tyr-271 and Tyr-299 is required for interaction with RASGAP.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Docking protein 2 56kDa antibody
    • Docking protein 2 antibody
    • DOK 2 antibody
    • DOK R antibody
    • DOK2 antibody
    • DOK2_HUMAN antibody
    • Downstream of tyrosine kinase 2 antibody
    • OTTHUMP00000161710 antibody
    • OTTHUMP00000224923 antibody
    • p56(dok 2) antibody
    • p56(dok-2) antibody
    • p56dok 2 antibody
    • p56DOK antibody
    • p56dok2 antibody
    see all

Anti-DOK2 antibody 画像

  • Ab1677 staining human breast carcinoma. Staining is localized to nucleus and cytoplasm.
    Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control.
    Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffers EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

Anti-DOK2 antibody (ab1677) 使用論文

ab1677 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (NMuMG)
Specification NMuMG
Fixative Paraformaldehyde
Permeabilization Yes - 0.1% Triton X100
Blocking step BSA as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C
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投稿 Jun 16 2008

Application Western blot
Sample Mouse Cell lysate - whole cell (NMuMG)
Loading amount 500000 cells
Specification NMuMG
Gel Running Conditions Reduced Denaturing (10%)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: RT°C
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投稿 Jun 03 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"