製品の概要

  • 製品名Anti-DOK1 (phospho Y398) antibody
    DOK1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to DOK1 (phospho Y398)
  • 特異性ab75741 detects endogenous levels of DOK1 only when phosphorylated at tyrosine 398.
  • アプリケーション適用あり: WB, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    A synthetic phosphopeptide derived from human DOK1 around the phosphorylation site of tyrosine 398 (E-G-YP-E-L).

  • ポジティブ・コントロール
    • Human breast carcinoma tissue. Extracts from K562 cells, starved.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab75741 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Predicted molecular weight: 52 kDa.
IHC-P 1/50 - 1/100.

ターゲット情報

  • 機能DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK1 appears to be a negative regulator of the insulin signaling pathway. Modulates integrin activation by competing with talin for the same binding site on ITGB3.
  • 組織特異性Expressed in pancreas, heart, leukocyte and spleen. Expressed in both resting and activated peripheral blood T-cells.
  • 配列類似性Belongs to the DOK family. Type A subfamily.
    Contains 1 IRS-type PTB domain.
    Contains 1 PH domain.
  • ドメインThe PTB domain mediates receptor interaction.
  • 翻訳後修飾Constitutively tyrosine-phosphorylated.
    Phosphorylated on tyrosine residues by the insulin receptor kinase. Results in the negative regulation of the insulin signaling pathway.
  • 細胞内局在Cytoplasm and Cytoplasm > perinuclear region.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Docking protein 1 62kD antibody
    • Docking protein 1 antibody
    • DOK 1 antibody
    • DOK1 antibody
    • DOK1_HUMAN antibody
    • Downstream of tyrosine kinase 1 antibody
    • p62(dok) antibody
    • P62DOK antibody
    • pp62 antibody
    see all

Anti-DOK1 (phospho Y398) antibody 画像

  • ab75741 at 1/50 dilution staining DOK1 in human breast carcinoma by Immunohistochemistry using paraffin-embedded tissue, in the absence or presence of the immunising phosphopeptide.
  • All lanes : Anti-DOK1 (phospho Y398) antibody (ab75741) at 1/500 dilution

    Lane 1 : K562 cell extracts, unstarved
    Lane 2 : K562 cell extracts, starved


    Predicted band size : 52 kDa
    Observed band size : 60 kDa (why is the actual band size different from the predicted?)

Anti-DOK1 (phospho Y398) antibody (ab75741) 使用論文

ab75741 has not yet been referenced specifically in any publications.

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