Anti-DOK1 (phospho Y398) 抗体 (ab75741)
Key features and details
- Rabbit polyclonal to DOK1 (phospho Y398)
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-DOK1 (phospho Y398) antibody
DOK1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to DOK1 (phospho Y398) -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide corresponding to Human DOK1 (phospho Y398).
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ポジティブ・コントロール
- Human breast carcinoma tissue. Extracts from K562 cells, starved.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+ -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
ab75741 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab75741の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/500 - 1/1000. Predicted molecular weight: 52 kDa.
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IHC-P |
1/50 - 1/100.
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特記事項 |
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WB
1/500 - 1/1000. Predicted molecular weight: 52 kDa. |
IHC-P
1/50 - 1/100. |
ターゲット情報
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機能
DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK1 appears to be a negative regulator of the insulin signaling pathway. Modulates integrin activation by competing with talin for the same binding site on ITGB3. -
組織特異性
Expressed in pancreas, heart, leukocyte and spleen. Expressed in both resting and activated peripheral blood T-cells. -
配列類似性
Belongs to the DOK family. Type A subfamily.
Contains 1 IRS-type PTB domain.
Contains 1 PH domain. -
ドメイン
The PTB domain mediates receptor interaction. -
翻訳後修飾
Constitutively tyrosine-phosphorylated.
Phosphorylated on tyrosine residues by the insulin receptor kinase. Results in the negative regulation of the insulin signaling pathway. -
細胞内局在
Cytoplasm and Cytoplasm > perinuclear region. - Information by UniProt
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参照データベース
- Entrez Gene: 1796 Human
- Omim: 602919 Human
- SwissProt: Q99704 Human
- Unigene: 103854 Human
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別名
- Docking protein 1 62kD antibody
- Docking protein 1 antibody
- DOK 1 antibody
see all
画像
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ab75741 at 1/50 dilution staining DOK1 in human breast carcinoma by Immunohistochemistry using paraffin-embedded tissue, in the absence or presence of the immunising phosphopeptide.
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All lanes : Anti-DOK1 (phospho Y398) antibody (ab75741) at 1/500 dilution
Lane 1 : K562 cell extracts, unstarved
Lane 2 : K562 cell extracts, starved
Predicted band size: 52 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab75741 は論文での使用が確認できていません。