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Synthetic peptide, corresponding to amino acids 425-439 of Human DOK1 (Peptide available as ab39756.)
Signals from most growth factors and cytokines are transduced by receptor tyrosine kinases or non-receptortyrosine kinases. Activated tyrosine kinases phosphorylatetheir substrates, which mediate the cellular response to extracellular stimuli. A long-sought major substrate termed p62dok (downstream of tyrosine kinase) for many tyrosine kinases including c-kit, v-abl, v-Fps, v-Src, v-Fms, and activated EGF, PDGF, IGF, VEGF and insulin receptors was identified recently from human and mouse by several laboratories. Upon phosphorylation, p62dok forms a complex with the ras GTPase-activating protein (RasGAP). p62dok represents a new family with very recently identified p56 dok.
Our Abpromise guarantee covers the use of ab8112 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 62 kDa.Can be blocked with Human DOK1 peptide (ab39756).|
|ICC/IF||Use a concentration of 2 µg/ml.|
|IP||Use at an assay dependent dilution. (see Abreview published on 27th July, 2006).|
ab8112 at 2µg/ml staining DOK1 in K562 cells by ICC/IF
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"