製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to DOK1
  • アプリケーション
    適用あり: WB, ICC/IF, IPmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide, corresponding to amino acids 425-439 of Human DOK1 (Peptide available as ab39756.)

  • ポジティブ・コントロール
    • Jurkat whole cell lysate
  • 特記事項
    Tyrosine kinase substrate p62DOK


    Signals from most growth factors and cytokines are transduced by receptor tyrosine kinases or non-receptortyrosine kinases. Activated tyrosine kinases phosphorylatetheir substrates, which mediate the cellular response to extracellular stimuli. A long-sought major substrate termed p62dok (downstream of tyrosine kinase) for many tyrosine kinases including c-kit, v-abl, v-Fps, v-Src, v-Fms, and activated EGF, PDGF, IGF, VEGF and insulin receptors was identified recently from human and mouse by several laboratories. Upon phosphorylation, p62dok forms a complex with the ras GTPase-activating protein (RasGAP). p62dok represents a new family with very recently identified p56 dok.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab8112 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 62 kDa.Can be blocked with Human DOK1 peptide (ab39756).
ICC/IF Use a concentration of 2 µg/ml.
IP Use at an assay dependent dilution. (see Abreview published on 27th July, 2006).

ターゲット情報

  • 機能
    DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK1 appears to be a negative regulator of the insulin signaling pathway. Modulates integrin activation by competing with talin for the same binding site on ITGB3.
  • 組織特異性
    Expressed in pancreas, heart, leukocyte and spleen. Expressed in both resting and activated peripheral blood T-cells.
  • 配列類似性
    Belongs to the DOK family. Type A subfamily.
    Contains 1 IRS-type PTB domain.
    Contains 1 PH domain.
  • ドメイン
    The PTB domain mediates receptor interaction.
  • 翻訳後修飾
    Constitutively tyrosine-phosphorylated.
    Phosphorylated on tyrosine residues by the insulin receptor kinase. Results in the negative regulation of the insulin signaling pathway.
  • 細胞内局在
    Cytoplasm and Cytoplasm > perinuclear region.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Docking protein 1 62kD antibody
    • Docking protein 1 antibody
    • DOK 1 antibody
    • DOK1 antibody
    • DOK1_HUMAN antibody
    • Downstream of tyrosine kinase 1 antibody
    • p62(dok) antibody
    • P62DOK antibody
    • pp62 antibody
    see all

画像

  • All lanes : Anti-DOK1 antibody (ab8112) at 1 µg/ml

    Lane 1 : Jurkat cell lysates
    Lane 2 : THP-1 cell lysate


    Observed band size : 62 kDa (why is the actual band size different from the predicted?)
  • ab8112 at 2µg/ml staining DOK1 in K562 cells by ICC/IF

参考文献

This product has been referenced in:
  • Friedrich T  et al. Subcellular compartmentalization of docking protein-1 contributes to progression in colorectal cancer. EBioMedicine 8:159-72 (2016). IHC . Read more (PubMed: 27428427) »
  • Siouda M  et al. Epstein-Barr virus down-regulates tumor suppressor DOK1 expression. PLoS Pathog 10:e1004125 (2014). WB . Read more (PubMed: 24809689) »

See all 7 Publications for this product

レビューと Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (Jurkat cells)
Total protein in input
2e+007 cells
Specification
Jurkat cells
Immuno-precipitation step
Protein A
Username

Mr. Guittard Geoffrey

Verified customer

投稿 Jul 27 2006

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

登録