製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to DOCK7
  • アプリケーション
    適用あり: ICC/IF, WB, IPmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Rabbit, Horse, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Platypus
  • 免疫原

    Synthetic peptide corresponding to a region within amino acids 175-225 of Human DOCK7 (UniProt Q96N67-4).

  • ポジティブ・コントロール
    • HeLa, 293T and Jurkat whole cell lysates. IF/ICC: U-87 MG cell line.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab118790 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 1 µg/ml.
WB 1/2000 - 1/10000. Predicted molecular weight: 243 kDa.
IP Use at 2-10 µg/mg of lysate.

ターゲット情報

  • 関連性
    DOCK7 functions as a guanine nucleotide exchange factor (GEF), which activates Rac1 and Rac3 Rho small GTPases by exchanging bound GDP for free GTP. It does not have a GEF activity for CDC42. It is required for STMN1 'Ser-15' phosphorylation during axon formation and consequently for neuronal polarization.
  • 細胞内局在
    Cell projection, axon. Note=Enriched in the developing axons of hippocampal neurons.
  • 参照データベース
  • 別名
    • 3110056M06Rik antibody
    • dedicator of cytokinesis 7 antibody
    • Dedicator of cytokinesis protein 7 antibody
    • Gm430 antibody
    • KIAA1771 antibody
    • MGC189434 antibody
    • mKIAA1771 antibody
    • RP23 329P19.2 antibody
    • ZIR2 antibody
    see all

画像

  • ICC/IF image of ab118790 stained U-87 MG cells. The cells were 4% fomaldeyhde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab118790, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • All lanes : Anti-DOCK7 antibody (ab118790) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : 293T whole cell lysate at 50 µg
    Lane 4 : Jurkat whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 243 kDa


    Exposure time : 30 seconds
  • Detection of DOCK7 in Immunoprecipitates of HeLa whole cell lysatse (1 mg for IP, 20% of IP loaded) using ab118790 at 6 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection.
    Detection: Chemiluminescence with an exposure time of 30 seconds.

参考文献

ab118790 has not yet been referenced specifically in any publications.

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