Rabbit polyclonal to DOCK10
Horse, Guinea pig, Cow, Dog, Chimpanzee, Rhesus monkey, Gorilla, Elephant
Synthetic peptide corresponding to a region between residue 100 and 150 of human DOCK10 (NP_055504.2)
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium Azide
Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8
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Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000. Predicted molecular weight: 250 kDa.
Use at 2-5 µg/mg of lysate.
DOCK10 is a potential guanine nucleotide exchange factor (GEF). GEF proteins activate some small GTPases by exchanging bound GDP for free GTP. Two named isoforms exist, produced by alternative splicing.
dedicator of cytokinesis 10 antibody
Western blot - Anti-DOCK10 antibody (ab75258)
All lanes : Anti-DOCK10 antibody (ab75258) at 0.1 µg/ml Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 5 µg Predicted band size: 250 kDa Observed band size: 250 kDa Additional bands at: 55 kDa. We are unsure as to the identity of these extra bands.
Immunoprecipitation - Anti-DOCK10 antibody (ab75258)
Detection of DOCK10 by Western Blot of Immunprecipitate.
ab75258 at 1µg/ml staining DOCK10 in HeLa whole cell lysate immunoprecipitated using ab75258 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded). Detection: chemiluminescence with exposure time of 1 minute.
has not yet been referenced specifically in any publications.
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