製品の概要

  • 製品名Anti-DOCK10 antibody
    DOCK10 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to DOCK10
  • アプリケーション適用あり: WB, IPmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Horse, Guinea pig, Cow, Dog, Chimpanzee, Rhesus monkey, Gorilla, Elephant
  • 免疫原

    Synthetic peptide corresponding to a region between residue 100 and 150 of human DOCK10 (NP_055504.2)

  • ポジティブ・コントロール
    • HeLa whole cell lysate.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: 0.09% Sodium Azide
    Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

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アプリケーション

Our Abpromise guarantee covers the use of ab75258 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000 - 1/10000. Predicted molecular weight: 250 kDa.
IP Use at 2-5 µg/mg of lysate.

ターゲット情報

  • 関連性DOCK10 is a potential guanine nucleotide exchange factor (GEF). GEF proteins activate some small GTPases by exchanging bound GDP for free GTP. Two named isoforms exist, produced by alternative splicing.
  • 参照データベース
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Anti-DOCK10 antibody 画像

  • All lanes : Anti-DOCK10 antibody (ab75258) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg


    Predicted band size : 250 kDa
    Observed band size : 250 kDa
    Additional bands at : 55 kDa. We are unsure as to the identity of these extra bands.
  • Detection of DOCK10 by Western Blot of Immunprecipitate.
    ab75258 at 1µg/ml staining DOCK10 in HeLa whole cell lysate immunoprecipitated using ab75258 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded).
    Detection: chemiluminescence with exposure time of 1 minute.

Anti-DOCK10 antibody (ab75258) 使用論文

ab75258 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"