製品の概要

  • 製品名
    Anti-DOCK1 antibody
    DOCK1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to DOCK1
  • 由来種
    Rabbit
  • アプリケーション
    適用あり: ICC/IF, WB, IHC-Pmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide corresponding to a region within amino acids 1852 and 1865 of Human DOCK1 (NP_001371).

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • ポジティブ･コントロール
    • WB: 293T whole cell lysate; HeLaS3 cell lysate ICC/IF: A431 cells IHC-P: Lung CA

法規制情報

医薬用外毒物

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab97325 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/100 - 1/200.
WB 1/500 - 1/3000. Predicted molecular weight: 215 kDa.
IHC-P 1/100 - 1/500.

ターゲット情報

  • 機能
    Involved in cytoskeletal rearrangements required for phagocytosis of apoptotic cells and cell motility. Functions as a guanine nucleotide exchange factor (GEF), which activates Rac Rho small GTPases by exchanging bound GDP for free GTP. Its GEF activity may be enhanced by ELMO1.
  • 組織特異性
    Highly expressed in placenta, lung, kidney, pancreas and ovary. Expressed at intermediate level in thymus, testes and colon.
  • 配列類似性
    Belongs to the DOCK family.
    Contains 1 DHR-1 (CZH-1) domain.
    Contains 1 DHR-2 (CZH-2) domain.
    Contains 1 SH3 domain.
  • ドメイン
    The DHR-2 domain is necessary and sufficient for the GEF activity.
  • 細胞内局在
    Cytoplasm. Membrane. Recruited to membranes via its interaction with phosphatidylinositol 3,4,5-triphosphate.
  • Information by UniProt
  • 参照データベース
    • 別名
      • 180 kDa protein downstream of CRK antibody
      • Ced5 antibody
      • Dedicator of Cytokinesis 1 antibody
      • Dedicator of cytokinesis protein 1 antibody
      • DOCK 1 antibody
      • DOCK 180 antibody
      • DOCK1 antibody
      • DOCK1_HUMAN antibody
      • DOCK180 antibody
      see all

    画像

    • Western blot - Anti-DOCK1 antibody (ab97325)
      Western blot - Anti-DOCK1 antibody (ab97325)
      Anti-DOCK1 antibody (ab97325) at 1/1000 dilution + 293T whole cell lysate at 30 µg

      Predicted band size: 215 kDa



      7.5% SDS Page
    • Immunocytochemistry/ Immunofluorescence - Anti-DOCK1 antibody (ab97325)
      Immunocytochemistry/ Immunofluorescence - Anti-DOCK1 antibody (ab97325)
      Immunofluorescence analysis of DOCK1 in methanol fixed A431, using ab97325 at a 1/200 dilution.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DOCK1 antibody (ab97325)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DOCK1 antibody (ab97325)
      Immunohistochemical analysis of DOCK1 in paraffin embedded Lung CA, using ab97325 at a 1/100 dilution.

    参考文献

    This product has been referenced in:
    • Toret CP  et al. An Elmo-Dock complex locally controls Rho GTPases and actin remodeling during cadherin-mediated adhesion. J Cell Biol 207:577-87 (2014). Read more (PubMed: 25452388) »

    See 1 Publication for this product

    ab97325 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

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