製品の概要
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製品名Anti-DOCK1 antibody
DOCK1 一次抗体 製品一覧 -
製品の詳細Rabbit polyclonal to DOCK1
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アプリケーション適用あり: ICC/IF, WB, IHC-Pmore details
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種交差性交差種: Human
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免疫原
Synthetic peptide corresponding to a region within amino acids 1852 and 1865 of Human DOCK1 (NP_001371).
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ポジティブ･コントロール
- WB: 293T whole cell lysate; HeLaS3 cell lysate ICC/IF: A431 cells IHC-P: Lung CA
法規制情報
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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バッファーPreservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol, 0.1% BSA, PBS, pH 7 -
Concentration information loading... -
精製度Immunogen affinity purified
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ポリ/モノポリクローナル
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アイソタイプIgG
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研究分野
関連製品
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Compatible Secondaries
アプリケーション
Our Abpromise guarantee covers the use of ab97325 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF | 1/100 - 1/200. | |
| WB | 1/500 - 1/3000. Predicted molecular weight: 215 kDa. | |
| IHC-P | 1/100 - 1/500. |
ターゲット情報
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機能Involved in cytoskeletal rearrangements required for phagocytosis of apoptotic cells and cell motility. Functions as a guanine nucleotide exchange factor (GEF), which activates Rac Rho small GTPases by exchanging bound GDP for free GTP. Its GEF activity may be enhanced by ELMO1.
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組織特異性Highly expressed in placenta, lung, kidney, pancreas and ovary. Expressed at intermediate level in thymus, testes and colon.
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配列類似性Belongs to the DOCK family.
Contains 1 DHR-1 (CZH-1) domain.
Contains 1 DHR-2 (CZH-2) domain.
Contains 1 SH3 domain. -
ドメインThe DHR-2 domain is necessary and sufficient for the GEF activity.
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細胞内局在Cytoplasm. Membrane. Recruited to membranes via its interaction with phosphatidylinositol 3,4,5-triphosphate.
- Information by UniProt
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参照データベース
- Entrez Gene: 1793 Human
- Omim: 601403 Human
- SwissProt: Q14185 Human
- Unigene: 159195 Human
- Unigene: 645702 Human
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別名
- 180 kDa protein downstream of CRK antibody
- Ced5 antibody
- Dedicator of Cytokinesis 1 antibody
see all
Anti-DOCK1 antibody 画像
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Western blot - DOCK1 antibody (ab97325)Anti-DOCK1 antibody (ab97325) at 1/1000 dilution + 293T whole cell lysate at 30 µg
Predicted band size : 215 kDa -
Immunocytochemistry/ Immunofluorescence - DOCK1 antibody (ab97325)Immunofluorescence analysis of DOCK1 in methanol fixed A431, using ab97325 at a 1/200 dilution. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - DOCK1 antibody (ab97325)Immunohistochemical analysis of DOCK1 in paraffin embedded Lung CA, using ab97325 at a 1/100 dilution.
プロトコール
Anti-DOCK1 antibody (ab97325) 使用論文
This product has been referenced in:
- Toret CP et al. An Elmo-Dock complex locally controls Rho GTPases and actin remodeling during cadherin-mediated adhesion. J Cell Biol 207:577-87 (2014). Read more (PubMed: 25452388) »
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"