製品の概要

  • 製品名Anti-DOCK1 antibody
    DOCK1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to DOCK1
  • アプリケーション適用あり: WB, IPmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide corresponding to a region between residues 1815 and 1865 of human DOCK1 (NP_001371.1)

  • ポジティブ・コントロール
    • HeLa, 293T and NIH3T3 whole cell lysates.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab76927 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
IP
  • 追加情報IP: Use at 2-5 µg/mg of lysate.
    WB: 1/2000 - 1/10,000. Predicted molecular weight: 215 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能Involved in cytoskeletal rearrangements required for phagocytosis of apoptotic cells and cell motility. Functions as a guanine nucleotide exchange factor (GEF), which activates Rac Rho small GTPases by exchanging bound GDP for free GTP. Its GEF activity may be enhanced by ELMO1.
    • 組織特異性Highly expressed in placenta, lung, kidney, pancreas and ovary. Expressed at intermediate level in thymus, testes and colon.
    • 配列類似性Belongs to the DOCK family.
      Contains 1 DHR-1 (CZH-1) domain.
      Contains 1 DHR-2 (CZH-2) domain.
      Contains 1 SH3 domain.
    • ドメインThe DHR-2 domain is necessary and sufficient for the GEF activity.
    • 細胞内局在Cytoplasm. Membrane. Recruited to membranes via its interaction with phosphatidylinositol 3,4,5-triphosphate.
    • Information by UniProt
    • 参照データベース
    • 別名
      • 180 kDa protein downstream of CRK antibody
      • Ced5 antibody
      • Dedicator of Cytokinesis 1 antibody
      • Dedicator of cytokinesis protein 1 antibody
      • DOCK 1 antibody
      • DOCK 180 antibody
      • DOCK1 antibody
      • DOCK1_HUMAN antibody
      • DOCK180 antibody
      see all

    Anti-DOCK1 antibody 画像

    • All lanes : Anti-DOCK1 antibody (ab76927) at 0.04 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg
      Lane 4 : 293T whole cell lysate at 50 µg
      Lane 5 : NIH3T3 whole cell lysate at 50 µg


      Predicted band size : 215 kDa
      Observed band size : 215 kDa


      Exposure time : 3 minutes
    • Detection of DOCK1 by Western Blot of Immunprecipitate.
      ab76927, at 1µg/ml, staining DOCK1 in HeLa whole cell lysate immunoprecipitated using ab76927 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
      Detection: Chemiluminescence with exposure time of 3 seconds.

    Anti-DOCK1 antibody (ab76927) 使用論文

    ab76927 has not yet been referenced specifically in any publications.

    Product Wall

    Application Immunoprecipitation
    Sample Human Cell lysate - whole cell (HeLa cell lines(cervical cancer))
    Total protein in input 30 µg
    Immuno-precipitation step Protein A/G
    Specification HeLa cell lines(cervical cancer)
    Username

    Abcam user community

    Verified customer

    投稿 Apr 25 2017

    Application Western blot
    Sample Human Cell lysate - whole cell (HeLa cell line (Cervical Cancer))
    Gel Running Conditions Reduced Denaturing (8%)
    Loading amount 30 µg
    Specification HeLa cell line (Cervical Cancer)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
    Username

    Abcam user community

    Verified customer

    投稿 Apr 25 2017

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"