The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at 2-5 µg/mg of lysate.
追加情報Is unsuitable for WB.
機能Involved in cytoskeletal rearrangements required for phagocytosis of apoptotic cells and cell motility. Functions as a guanine nucleotide exchange factor (GEF), which activates Rac Rho small GTPases by exchanging bound GDP for free GTP. Its GEF activity may be enhanced by ELMO1.
組織特異性Highly expressed in placenta, lung, kidney, pancreas and ovary. Expressed at intermediate level in thymus, testes and colon.
配列類似性Belongs to the DOCK family. Contains 1 DHR-1 (CZH-1) domain. Contains 1 DHR-2 (CZH-2) domain. Contains 1 SH3 domain.
ドメインThe DHR-2 domain is necessary and sufficient for the GEF activity.
細胞内局在Cytoplasm. Membrane. Recruited to membranes via its interaction with phosphatidylinositol 3,4,5-triphosphate.
Detection of DOCK1 by Western Blot of Immunprecipitate.
anti-DOCK1 at 1µg/ml staining DOCK1 in HeLa whole cell lysate immunoprecipitated using ab75278 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 3 seconds.
Anti-DOCK1 antibody (ab75278) 使用論文
has not yet been referenced specifically in any publications.