Anti-Dnmt3L 抗体 [N117/9] (ab93613)

製品の概要

  • 製品名
    Anti-Dnmt3L antibody [N117/9]
    Dnmt3L 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [N117/9] to Dnmt3L
  • 特異性
    Detects Dnmt3L, it does not react with DNMT3a or DNMT3B.
  • アプリケーション
    適用あり: WB, IHC-P, ICC/IF, IHC-Frmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Fusion protein, corresponding to amino acids 1-387 of Human Dnmt3L (accession number BAA95556)

  • 特記事項

    The clone number has been updated from S117-9 to N117/9, both clone numbers name the same antibody clone.

     

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: 0.09% Sodium Azide
    Constituents: 50% Glycerol, PBS, pH 7.4
  • Concentration information loading...
  • 精製度
    Protein G purified
  • ポリ/モノ
    モノクローナル
  • クローン名
    N117/9
  • アイソタイプ
    IgG1
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab93613 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
IHC-P
ICC/IF
IHC-Fr
  • 追加情報
    ICC/IF: Use at a concentration of 1 - 10 µg/ml.
    IHC-P: Use at a concentration of 0.1 - 1 µg/ml.
    IHC-Fr: Use at a concentration of 0.1 - 1 µg/ml.
    WB: Use at a concentration of 1 - 10 µg/ml. Predicted molecular weight: 44 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能
      Catalytically inactive regulatory factor of DNA methyltransferases. It is essential for the function of DNMT3A and DNMT3B. Activates DNMT3A and DNMT3B by binding to their catalytic domain. Accelerates the binding of DNA and AdoMet to the methyltransferases and dissociates from the complex after DNA binding to the methyltransferases. Recognizes unmethylated histone H3 lysine 4 (H3K4) and induces de novo DNA methylation by recruitment or activation of DNMT3.
    • 組織特異性
      Expressed at low levels in several tissues including testis, ovary, and thymus.
    • 配列類似性
      Belongs to the C5-methyltransferase family.
      Contains 1 ADD-type zinc finger.
    • 細胞内局在
      Nucleus.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Cytosine 5 methyltransferase 3 like protein antibody
      • DNA (cytosine 5 ) methyltransferase 3 like antibody
      • DNA (cytosine-5)-methyltransferase 3-like antibody
      • DNA cytosine 5 methyltransferase 3 like protein antibody
      • DNA methyltransferase 3 like protein antibody
      • DNM3L_HUMAN antibody
      • Dnmt 3L antibody
      • Dnmt3l antibody
      • Human cytosine 5 methyltransferase 3 like protein antibody
      • MGC1090 antibody
      see all

    画像

    • Immunohistochemical analysis of paraffin-embedded Mouse backskin labeling Dnmt3L with ab93613 at 1 ug/ml

    • Immunofluorescent analysis on Human HaCaT cells labeling Dnmt3L with ab93613 at 1/1000 dilution.



    • Predicted band size : 44 kDa
      Western blot detection of DNMT3L in a Human tissue mix using ab93613 at 1:1000 dilution.

    参考文献

    ab93613 has not yet been referenced specifically in any publications.

    レビューと Q&A

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Dako antigen retrieval solution
    Sample
    Marmoset (common) Tissue sections (adult testis)
    Specification
    adult testis
    Permeabilization
    No
    Fixative
    Formaldehyde
    Username

    煜欽 林

    Verified customer

    投稿 Jul 17 2013

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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