製品の概要

  • 製品名Anti-Dnmt3b antibody
    Dnmt3b 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Dnmt3b
  • アプリケーション適用あり: IHC-P, WBmore details
  • 種交差性
    交差種: Mouse, Chinese Hamster
    交差が予測される動物種: Rat, Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Mouse Dnmt3b.

    (Peptide available as ab16376.)

  • ポジティブ・コントロール
    • This antibody gave a positive result in the following whole cell lysates: CHO, HUES7, F9, Mouse Embryonic Germ Cell, E14Tg2a.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab16049 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use at an assay dependent concentration.
WB Use a concentration of 0.8 - 1 µg/ml. Detects a band of approximately 110 kDa (predicted molecular weight: 100 kDa). Abcam recommends using milk as the blocking agent (3-5%). Please contact Scientific Support for more information.

ターゲット情報

  • 機能Required for genome wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. May preferentially methylates nucleosomal DNA within the nucleosome core region. May function as transcriptional co-repressor by associating with CBX4 and independently of DNA methylation. Seems to be involved in gene silencing (By similarity). In association with DNMT1 and via the recruitment of CTCFL/BORIS, involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9. Isoforms 4 and 5 are probably not functional due to the deletion of two conserved methyltransferase motifs.
  • 組織特異性Ubiquitous; highly expressed in fetal liver, heart, kidney, placenta, and at lower levels in spleen, colon, brain, liver, small intestine, lung, peripheral blood mononuclear cells, and skeletal muscle. Isoform 1 is expressed in all tissues except brain, skeletal muscle and PBMC, 3 is ubiquitous, 4 is expressed in all tissues except brain, skeletal muscle, lung and prostate and 5 is detectable only in testis and at very low level in brain and prostate.
  • 関連疾患Defects in DNMT3B are a cause of immunodeficiency-centromeric instability-facial anomalies syndrome (ICF) [MIM:242860]. ICF is a rare autosomal recessive disorder characterized by a variable immunodeficiency, mild facial anomalies, and centromeric heterochromatin instability involving chromosomes 1, 9, and 16. ICF is biochemically characterized by hypomethylation of CpG sites in some regions of heterochromatin.
  • 配列類似性Belongs to the C5-methyltransferase family.
    Contains 1 ADD domain.
    Contains 1 GATA-type zinc finger.
    Contains 1 PHD-type zinc finger.
    Contains 1 PWWP domain.
  • ドメインThe PWWP domain is essential for targeting to pericentric heterochromatin.
  • 翻訳後修飾Sumoylated.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Cytosine 5methyltransferase 3B antibody
    • DNA antibody
    • DNA (cytosine 5) methyltransferase 3 beta antibody
    • DNA (cytosine 5)-methyltransferase 3B antibody
    • DNA (cytosine-5)-methyltransferase 3B antibody
    • DNA methyltransferase HsaIIIB antibody
    • DNA MTase HsaIIIB antibody
    • DNM3B_HUMAN antibody
    • Dnmt3b antibody
    • EC 2.1.1.37 antibody
    • ICF antibody
    • ICF1 antibody
    • M.HsaIIIB antibody
    • MGC124407 antibody
    • RP23-89H14.3 antibody
    see all

Anti-Dnmt3b antibody 画像

  • All lanes : Anti-Dnmt3b antibody (ab16049) at 1 µg/ml (blocked with 3% milk.)

    Lane 1 : HUES7 (Human embryonic stem cell line) Whole Cell Lysate
    Lane 2 : F9 (Mouse embryonic carcinoma cell line) Whole Cell Lysate
    Lane 3 : Mouse Embryonic Germ Cell Lysate
    Lane 4 : E14Tg2a (Mouse embryonic stem cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Performed under reducing conditions.

    Predicted band size : 100 kDa


    Exposure time : 2 minutes
  • All lanes : Anti-Dnmt3b antibody (ab16049) at 1 µg/ml

    Lane 1 : CHO cell lysate
    Lane 2 : CHO cell lysate with Mouse Dnmt3b peptide (ab16376) at 1 µg/ml

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat anti-rabbit Alexa fluor 680 at 1/5000 dilution

    Performed under reducing conditions.

    Predicted band size : 100 kDa
  • All lanes : Anti-Dnmt3b antibody (ab16049) at 1/1000 dilution

    Lane 1 : Lysate prepared from human NCCIT cell chromatin fraction
    Lane 2 : Lysate prepared from chromatin fraction of human NCCIT whole cells

    Lysates/proteins at 200000 cells per lane.

    Secondary
    HRP-conjugated goat polyclonal to rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 100 kDa
    Observed band size : 100 kDa
    Additional bands at : 110 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 seconds

    This image is a courtesy of Johanna Smith

    See Abreview

Anti-Dnmt3b antibody (ab16049) 使用論文

This product has been referenced in:
  • Sharma S  et al. Secreted Protein Acidic and Rich in Cysteine (SPARC) Mediates Metastatic Dormancy of Prostate Cancer in Bone. J Biol Chem 291:19351-63 (2016). Read more (PubMed: 27422817) »
  • Yan MD  et al. Fucoidan Elevates MicroRNA-29b to Regulate DNMT3B-MTSS1 Axis and Inhibit EMT in Human Hepatocellular Carcinoma Cells. Mar Drugs 13:6099-116 (2015). WB ; Human . Read more (PubMed: 26404322) »

See all 8 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Testis)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid pH6
Permeabilization No
Specification Testis
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Fixative Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

投稿 Dec 14 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (Testis)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization No
Specification Testis
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Fixative Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

投稿 Dec 14 2015

Abcam has not validated the combination of species/application used in this Abreview.
Application IHC - Wholemount
Sample Rat Tissue (Dorsal Root Ganglia)
Specification Dorsal Root Ganglia
Username

Sarah Pollema

Verified customer

投稿 Oct 16 2012

DISCOUNT CODE: ****
Expiration date: January 21, 2013

I am very pleased to hear you would like to accept our offer and test ab16049 on rat tissue. This code will give you one free primary antibody before the expiration date. To redeem t...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Ovary)
Specification Ovary
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Dako Target Retreival Solution
Permeabilization No
Blocking step 5 minutes of peroxidase block then 10 minutes of protein block. These are ready-to-use reagents purchased from Dako as blocking agent for 15 minute(s) · Concentration: 100% · Temperature: 20°C
Username

Mr. Antibody Solutions

Verified customer

投稿 May 25 2010

Application Western blot
Sample Human Cell lysate - other (NCCIT cells - mediastinal mixed germ cell tumor)
Loading amount 200000 cells
Specification NCCIT cells - mediastinal mixed germ cell tumor
Gel Running Conditions Reduced Denaturing (7.5%)
Blocking step Milk as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Ms. Johanna Smith

Verified customer

投稿 Aug 28 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"