Anti-Dnmt3a (phospho S390 + S393) 抗体 (ab87763)
製品の概要
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製品名Anti-Dnmt3a (phospho S390 + S393) antibody
Dnmt3a 一次抗体 製品一覧 -
製品の詳細Rabbit polyclonal to Dnmt3a (phospho S390 + S393)
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由来種Rabbit
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アプリケーション適用あり: ICC/IF, WBmore details
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種交差性交差種: Human
交差が予測される動物種: Rat, Sheep, Rabbit, Horse, Cow, Dog, Pig, Chimpanzee, Macaque monkey, Gorilla, Orangutan
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免疫原
Synthetic peptide conjugated to KLH derived from within residues 350 - 450 of Human Dnmt3a, phosphorylated at S390 and phosphorylated at S393.
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ポジティブ･コントロール
- This antibody gave a positive signal in HEK293 whole cell lysate. This antibody gave a positive signal in the following methanol fixed cell lines: HepG2.
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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バッファーpH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
精製度Immunogen affinity purified
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ポリ/モノポリクローナル
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アイソタイプIgG
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研究分野
関連製品
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Compatible Secondaries
アプリケーション
Our Abpromise guarantee covers the use of ab87763 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF | Use a concentration of 5 µg/ml. | |
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 120 kDa (predicted molecular weight: 102 kDa). |
ターゲット情報
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機能Required for genome wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. It modifies DNA in a non-processive manner and also methylates non-CpG sites. May preferentially methylate DNA linker between 2 nucleosomal cores and is inhibited by histone H1. Plays a role in paternal and maternal imprinting. Required for methylation of most imprinted loci in germ cells. Acts as a transcriptional corepressor for ZNF238. Can actively repress transcription through the recruitment of HDAC activity.
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組織特異性Highly expressed in fetal tissues, skeletal muscle, heart, peripheral blood mononuclear cells, kidney, and at lower levels in placenta, brain, liver, colon, spleen, small intestine and lung.
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配列類似性Belongs to the C5-methyltransferase family.
Contains 1 ADD domain.
Contains 1 GATA-type zinc finger.
Contains 1 PHD-type zinc finger.
Contains 1 PWWP domain. -
ドメインThe PWWP domain is essential for targeting to pericentric heterochromatin.
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翻訳後修飾Sumoylated; sumoylation disrupts the ability to interact with histone deacetylases (HDAC1 and HDAC2) and repress transcription.
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細胞内局在Nucleus. Cytoplasm. Accumulates in the major satellite repeats at pericentric heterochromatin.
- Information by UniProt
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参照データベース
- Entrez Gene: 359716 Cow
- Entrez Gene: 482996 Dog
- Entrez Gene: 1788 Human
- Entrez Gene: 444984 Rat
- Omim: 602769 Human
- SwissProt: Q9Y6K1 Human
- SwissProt: Q1LZ53 Rat
- Unigene: 515840 Human
see all -
別名
- DNA (cytosine 5) methyltransferase 3 alpha antibody
- DNA (cytosine 5) methyltransferase 3A antibody
- DNA (cytosine-5)-methyltransferase 3A antibody
see all
画像
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Western blot - Anti-Dnmt3a (phospho S390 + S393) antibody (ab87763)All lanes : Anti-Dnmt3a (phospho S390 + S393) antibody (ab87763) at 1 µg/ml
Lane 1 : HEK293 (Human) Whole Cell Lysate (ab52256)
Lane 2 : HEK293 (Human) Whole Cell Lysate (ab52256) with Phospho S390 + S393 peptide at 1 µg/ml
Lane 3 : HEK293 (Human) Whole Cell Lysate (ab52256) with Phospho S390 peptide at 1 µg/ml
Lane 4 : HEK293 (Human) Whole Cell Lysate (ab52256) with Phospho S393 peptide at 1 µg/ml
Lane 5 : HEK293 (Human) Whole Cell Lysate (ab52256) with Non modified control peptide at 1 µg/ml
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 102 kDa
Observed band size: 120 kDa (why is the actual band size different from the predicted?)
Additional bands at: 39 kDa, 52 kDa, 68 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes -
Western blot - Anti-Dnmt3a (phospho S390 + S393) antibody (ab87763)Lysates from U2OS WT cells were loaded as input. The western blot was probed with anti Dnmt3a (phospho S390 + S393) antibody (ab87763) either in the presence or absence of double modified peptide phosphorylated on S390 and S393 as indicated in the figure. Detected phospho Dnmt3a is indicated by the arrow. Please note: the observed band migrates at a higher MW than predicted probably because of phosphorylation. This data was provided by an anonymous collaborator. -
Immunocytochemistry/ Immunofluorescence - Anti-Dnmt3a (phospho S390 + S393) antibody (ab87763)ICC/IF image of ab87763 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab87763 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
参考文献
This product has been referenced in:
- Deplus R et al. Regulation of DNA methylation patterns by CK2-mediated phosphorylation of Dnmt3a. Cell Rep 8:743-53 (2014). WB ; Mouse, Human . Read more (PubMed: 25066127) »
レビューと Q&A
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