The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
EMSA: 2ul antiserum is used in PMID 19046370.
WB: 1/1000. Detects a band of approximately 85 kDa.
Is unsuitable for Neut.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Required for genome wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. It modifies DNA in a non-processive manner and also methylates non-CpG sites. May preferentially methylate DNA linker between 2 nucleosomal cores and is inhibited by histone H1. Plays a role in paternal and maternal imprinting. Required for methylation of most imprinted loci in germ cells. Acts as a transcriptional corepressor for ZNF238. Can actively repress transcription through the recruitment of HDAC activity.
Highly expressed in fetal tissues, skeletal muscle, heart, peripheral blood mononuclear cells, kidney, and at lower levels in placenta, brain, liver, colon, spleen, small intestine and lung.
Yossifoff M et al. Dynamic changes in DNA methylation during thermal control establishment affect CREB binding to the brain-derived neurotrophic factor promoter. Eur J Neurosci28:2267-77 (2008).
Read more (PubMed: 19046370) »
Loriot A et al. Transient down-regulation of DNMT1 methyltransferase leads to activation and stable hypomethylation of MAGE-A1 in melanoma cells. J Biol Chem281:10118-26 (2006).
Read more (PubMed: 16497664) »